Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ Pubmed: 38326651 DOI: doi:10.1038/s41594-024-01223-8 ]
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Cholesterol (386 Da, Ligand)
Structure of CX3CL1-US28-G11iN18-scFv16 in TL-state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7rkf
Q-score: 0.44
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- G11in18 heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein subunit alpha-11 (41 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-g11in18-scfv16 complex (Complex)
- Scfv16 (30 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
Structure of CX3CL1-US28-Gi-scFv16 in C-state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rkm
Q-score: 0.477
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human betaherpesvirus 5)
Structure of CX3CL1-US28-Gi-scFv16 in OC-state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7rkn
Q-score: 0.447
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
Structure of US27-Gi-scFv16 in CL-state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rkx
Q-score: 0.529
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Us27-gi-scfv16 complex (Complex)
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
Binding mode of US27-Gi-scFv16 in OCL-state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7rky
Q-score: 0.43
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Us27-gi-scfv16 complex (Complex)
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)