cryo-EM structure of GPR6-Gs-Nb35 complex
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8tyw
Q-score: 0.398
Barekatain M, Johansson LC, Lam JH, Chang H, Sadybekov AV, Han GW, Russo J, Bliesath J, Brice NL, Carlton MBL, Saikatendu KS, Sun H, Murphy ST, Monenschein H, Schiffer HH, Popov P, Lutomski CA, Robinson CV, Liu ZJ, Hua T, Katritch V, Cherezov V
Sci Signal (2024) 17 pp. eado8741-eado8741 [ Pubmed: 39626010 DOI: doi:10.1126/scisignal.ado8741 ]
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpr6-gs-nb35 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 6 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Orphan GPR20 in complex with Fab046
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8hs2
Q-score: 0.479
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Soluble cytochrome b562,g-protein coupled receptor 20 (50 kDa, Protein from Homo sapiens)
- Heavy chain of fab046 (26 kDa, Protein from Rattus)
- The complex of gpr20 with fab046 (Complex from Homo sapiens)
- Light chain of fab046 (25 kDa, Protein from Rattus)
Gi bound orphan GPR20 in ligand-free state
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8hs3
Q-score: 0.483
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Scfv16 (31 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Ggama (8 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
- Orphan gpr20 complex with gi trimer in the absence of a ligand (Complex from Homo sapiens)
Gi bound Orphan GPR20 complex with Fab046 in ligand-free state
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8hsc
Q-score: 0.467
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Light chain of fab046 (25 kDa, Protein from Rattus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (31 kDa, Protein from Vicugna pacos)
- The complex of gi bound orphan gpr20 with fab046 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Heavy chain of fab046 (25 kDa, Protein from Rattus)
cryo-EM structure of GPR52-miniGs-NB35
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 6li3
Q-score: 0.379
Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J, Lei M, Xu F
Nature (2020) 579 pp. 152-157 [ DOI: doi:10.1038/s41586-020-2019-0 Pubmed: 32076264 ]
- Gpr52-minigs-nb35 (Complex)
- Gsalpha-nb35 (Complex from Homo sapiens)
- Nanobody nb35 (16 kDa, Protein from Lama glama)
- Gpr52-gsbeta-gsgamma (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 52 (39 kDa, Protein from Homo sapiens)