Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ Pubmed: 38326651 DOI: doi:10.1038/s41594-024-01223-8 ]
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of an orphan GPCR-Gi protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8g05
Q-score: 0.524
Zhang X, Wang Y, Supekar S, Cao X, Zhou J, Dang J, Chen S, Jenkins L, Marsango S, Li X, Liu G, Milligan G, Feng M, Fan H, Gong W, Zhang C
Nat Commun (2023) 14 pp. 5706-5706 [ Pubmed: 37709767 DOI: doi:10.1038/s41467-023-41201-0 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 6-(octylamino)pyrimidine-2,4(3h,5h)-dione (239 Da, Ligand)
- 6oau_gpr84_dngi_scfv16 (Complex from Spodoptera frugiperda)
- G-protein coupled receptor 84 (48 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8kh5
Q-score: 0.514
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Cryo-em structure of the gpr174 and mini-gs complex with nb35 (Complex)
- Probable g-protein coupled receptor 174 (39 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- O-{hydroxy[((2r)-2-hydroxy-3-{[(1s)-1-hydroxypentadecyl]oxy}propyl)oxy]phosphoryl}-l-serine (485 Da, Ligand)
- Gs complex (Complex from Homo sapiens)
- Gpr174 (Complex from Homo sapiens)
Cryo-EM structure of the GPR161-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8kh4
Q-score: 0.48
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr161 and mini-gs complex with nb35 (Complex from Homo sapiens)
- G-protein coupled receptor 161 (41 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8tb7
Q-score: 0.517
Lees JA, Dias JM, Rajamohan F, Fortin JP, O'Connor R, Kong JX, Hughes EAG, Fisher EL, Tuttle JB, Lovett G, Kormos BL, Unwalla RJ, Zhang L, Dechert Schmitt AM, Zhou D, Moran M, Stevens KA, Fennell KF, Varghese AE, Maxwell A, Cote EE, Zhang Y, Han S
Nat Commun (2023) 14 pp. 5938-5938 [ DOI: doi:10.1038/s41467-023-41646-3 Pubmed: 37741852 ]
- Water (18 Da, Ligand)
- Fab hinge-binding nanobody (13 kDa, Protein from Lama glama)
- G-protein coupled receptor 61,soluble cytochrome b562 (59 kDa, Protein from Homo sapiens)
- Gpr61-icl3-bril chimera in complex with anti-bril fab24 bak5 and hinge-binding nanobody bound to inverse agonist compound 1 (Complex from Homo sapiens)
- Fab24 bak5 heavy chain (52 kDa, Protein from Homo sapiens)
- 6-{[(3,5-difluoropyridin-4-yl)methyl]amino}-n-(4-ethoxy-6-methylpyrimidin-2-yl)-2-methoxy-n-(2-methoxyethyl)pyridine-3-sulfonamide (524 Da, Ligand)
- Fab24 bak5 light chain (23 kDa, Protein from Homo sapiens)
Cryo-EM structure of the 3-OH-C12-bound GPR84 receptor-Gi complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8j18
Q-score: 0.499
Liu H, Zhang Q, He X, Jiang M, Wang S, Yan X, Cheng X, Liu Y, Nan FJ, Xu HE, Xie X, Yin W
Nat Commun (2023) 14 pp. 3271-3271 [ Pubmed: 37277332 DOI: doi:10.1038/s41467-023-38985-6 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 84 (45 kDa, Protein from Homo sapiens)
- (3r)-3-hydroxydodecanoic acid (216 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 3-oh-c12-bound gpr84 receptor-gi complex (Complex from Homo sapiens)
- Antibody fragment scfv16 (28 kDa, Protein from synthetic construct)
Lodoxamide-bound GPR35 in complex with G13
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8h8j
Q-score: 0.416
Duan J, Liu Q, Yuan Q, Ji Y, Zhu S, Tan Y, He X, Xu Y, Shi J, Cheng X, Jiang H, Eric Xu H, Jiang Y
Cell Discov (2022) 8 pp. 135-135 [ DOI: doi:10.1038/s41421-022-00499-8 Pubmed: 36543774 ]
- Lodoxamide-gpr35-g13 complex (Complex from Homo sapiens)
- G-protein coupled receptor 35 (33 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- 2,2'-[(2-chloro-5-cyano-1,3-phenylene)bis(azanediyl)]bis(oxoacetic acid) (311 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein subunit alpha-13 (42 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7xkd
Q-score: 0.563
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ Pubmed: 35982227 DOI: doi:10.1038/s41589-022-01084-6 ]
- Nb35 (Complex from Mus musculus)
- Adhesion g-protein coupled receptor g2 (Complex)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Adhesion g-protein (Complex from Camelus bactrianus)
- Adhesion g-protein coupled receptor g2 (130 kDa, Complex from Homo sapiens)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (34 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Cryo-EM structure of DHEA-ADGRG2-FL-Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7xke
Q-score: 0.532
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ Pubmed: 35982227 DOI: doi:10.1038/s41589-022-01084-6 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (220 kDa, Complex from Mus musculus)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Adhesion g-protein coupled receptor g2 (98 kDa, Protein from Mus musculus)
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex at lower state
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7xkf
Q-score: 0.563
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ Pubmed: 35982227 DOI: doi:10.1038/s41589-022-01084-6 ]
- Nb35 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Adhesion g-protein (Complex from Camelus bactrianus)
- Adhesion g-protein coupled receptor g2 (130 kDa, Complex from Homo sapiens)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (34 kDa, Protein from Mus musculus)
- Receptor g2 (Complex)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)