Cryo-EM structure of the GPR34 receptor in complex with the antagonist YL-365
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 8iyx
Q-score: 0.448
Xia A, Yong X, Zhang C, Lin G, Jia G, Zhao C, Wang X, Hao Y, Wang Y, Zhou P, Yang X, Deng Y, Wu C, Chen Y, Zhu J, Tang X, Liu J, Zhang S, Zhang J, Xu Z, Hu Q, Zhao J, Yue Y, Yan W, Su Z, Wei Y, Zhou R, Dong H, Shao Z, Yang S
PNAS (2023) 120 pp. e2308435120-e2308435120 [ Pubmed: 37733739 DOI: doi:10.1073/pnas.2308435120 ]
- Cryo-em structure of the gpr34 receptor in complex with the antagonist yl-365 (Complex from Homo sapiens)
- 1-[4-(3-chlorophenyl)phenyl]carbonyl-4-[2-(4-phenylmethoxyphenyl)ethanoylamino]piperidine-4-carboxylic acid (583 Da, Ligand)
- Probable g-protein coupled receptor 34,probable g-protein coupled receptor 34,yl-365 (66 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8hj1
Q-score: 0.445
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (39 kDa, Protein from Homo sapiens)
- Gpr21(wt) with gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
Cryo-EM structure of GPR34-Gi complex
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8sai
Q-score: 0.471
Xia A, Yong X, Zhang C, Lin G, Jia G, Zhao C, Wang X, Hao Y, Wang Y, Zhou P, Yang X, Deng Y, Wu C, Chen Y, Zhu J, Tang X, Liu J, Zhang S, Zhang J, Xu Z, Hu Q, Zhao J, Yue Y, Yan W, Su Z, Wei Y, Zhou R, Dong H, Shao Z, Yang S
PNAS (2023) 120 pp. e2308435120-e2308435120 [ Pubmed: 37733739 DOI: doi:10.1073/pnas.2308435120 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Soluble cytochrome b562,probable g-protein coupled receptor 34, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i) subunit alpha-1, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Human gpr34 in complex with gi heterotrimer and scfv16 (Complex)
- Probable g-protein coupled receptor 34 (53 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- O-[(s)-hydroxy{[(2s)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-l-serine (523 Da, Ligand)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8hix
Q-score: 0.489
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Gpr21 and gs protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8hj2
Q-score: 0.425
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gpr21(wt) with g15 (Complex from Homo sapiens)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8hj0
Q-score: 0.466
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gpr21 m5 with g15 (Complex from Homo sapiens)
- Probable g-protein coupled receptor 21 (38 kDa, Protein from Homo sapiens)
Orphan GPR20 in complex with Fab046
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8hs2
Q-score: 0.479
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Soluble cytochrome b562,g-protein coupled receptor 20 (50 kDa, Protein from Homo sapiens)
- Heavy chain of fab046 (26 kDa, Protein from Rattus)
- The complex of gpr20 with fab046 (Complex from Homo sapiens)
- Light chain of fab046 (25 kDa, Protein from Rattus)
Gi bound orphan GPR20 in ligand-free state
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8hs3
Q-score: 0.483
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Scfv16 (31 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Ggama (8 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
- Orphan gpr20 complex with gi trimer in the absence of a ligand (Complex from Homo sapiens)
Gi bound Orphan GPR20 complex with Fab046 in ligand-free state
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8hsc
Q-score: 0.467
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ Pubmed: 36849514 DOI: doi:10.1038/s41421-023-00520-8 ]
- Light chain of fab046 (25 kDa, Protein from Rattus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (31 kDa, Protein from Vicugna pacos)
- The complex of gi bound orphan gpr20 with fab046 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Heavy chain of fab046 (25 kDa, Protein from Rattus)
cryo-EM structure of GPR52-miniGs-NB35
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 6li3
Q-score: 0.379
Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J, Lei M, Xu F
Nature (2020) 579 pp. 152-157 [ DOI: doi:10.1038/s41586-020-2019-0 Pubmed: 32076264 ]
- Gpr52-minigs-nb35 (Complex)
- Gsalpha-nb35 (Complex from Homo sapiens)
- Nanobody nb35 (16 kDa, Protein from Lama glama)
- Gpr52-gsbeta-gsgamma (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 52 (39 kDa, Protein from Homo sapiens)