Structure of Src in complex with beta-arrestin 1 revealing SH3 binding sites
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9bt8
Q-score: 0.539
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Proto-oncogene tyrosine-protein kinase src (54 kDa, Protein from Gallus gallus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Bacteriophage sp.)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Bacteriophage sp.)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with three-domain src (sh3-sh2-sh1). (Complex from Rattus norvegicus)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9cx3
Q-score: 0.423
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cx9
Q-score: 0.47
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Lysophosphatidylserine receptor GPR34-Gi complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8iz4
Q-score: 0.52
Gong W, Liu G, Li X, Wang Y, Zhang X
To Be Published
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- [(2~{r})-2-oxidanyl-3-phosphonooxy-propyl] octadecanoate (438 Da, Ligand)
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 34 (64 kDa, Protein from Homo sapiens)
- Lysops-gpr34-gi-scfv16 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
GPR3 Orphan G-coupled Protein Receptor in complex with Dominant Negative Gs.
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8u8f
Q-score: 0.422
Russell IC, Zhang X, Bumbak F, McNeill SM, Josephs TM, Leeming MG, Christopoulos G, Venugopal H, Flocco MM, Sexton PM, Wootten D, Belousoff MJ
Biochemistry (2024) 63 pp. 625-631 [ DOI: doi:10.1021/acs.biochem.3c00647 Pubmed: 38376112 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Active state, gpr3 complex with dominant negative g-alphas. (Complex from Homo sapiens)
- G-protein coupled receptor 3 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Lysophosphatidylserine receptor GPR34-Gi complex
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8wrb
Q-score: 0.545
Liu G, Li X, Wang Y, Zhang X, Gong W
PLoS Biol (2023) 21 pp. e3002387-e3002387 [ DOI: doi:10.1371/journal.pbio.3002387 Pubmed: 38048360 ]
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- [(2r)-2-oxidanyl-3-phosphonooxy-propyl] (z)-octadec-9-enoate (436 Da, Ligand)
- Lysops-gpr34-gi-scfv16 complex (Complex from Homo sapiens)
- Probable g-protein coupled receptor 34,tag (64 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of an orphan GPCR-Gi protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8g05
Q-score: 0.524
Zhang X, Wang Y, Supekar S, Cao X, Zhou J, Dang J, Chen S, Jenkins L, Marsango S, Li X, Liu G, Milligan G, Feng M, Fan H, Gong W, Zhang C
Nat Commun (2023) 14 pp. 5706-5706 [ Pubmed: 37709767 DOI: doi:10.1038/s41467-023-41201-0 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 6-(octylamino)pyrimidine-2,4(3h,5h)-dione (239 Da, Ligand)
- 6oau_gpr84_dngi_scfv16 (Complex from Spodoptera frugiperda)
- G-protein coupled receptor 84 (48 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Lysophosphatidylserine receptor GPR174-Gs complex
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8izb
Q-score: 0.51
Liu G, Li X, Wang Y, Zhang X, Gong W
PLoS Biol (2023) 21 pp. e3002387-e3002387 [ DOI: doi:10.1371/journal.pbio.3002387 Pubmed: 38048360 ]
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Lysops-bound gpr174 in complex with gs and nb35. (Complex from Homo sapiens)
- [(2r)-2-oxidanyl-3-phosphonooxy-propyl] (z)-octadec-9-enoate (436 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Probable g-protein coupled receptor 174 (63 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from synthetic construct)