CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-2825
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 9dqh
Q-score: 0.123
Wang C, Liu Y, Lanier M, Yeager A, Singh I, Gumpper RH, Krumm BE, DeLeon C, Zhang S, Boehm M, Pittner R, Baron A, Dvorak L, Bacon C, Shoichet BK, Martinborough E, Fay JF, Cao C, Roth BL
Cell Rep (2024) 43 pp. 114942-114942 [ DOI: doi:10.1016/j.celrep.2024.114942 Pubmed: 39580805 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ep-2825-bound mrgprd-gq complex (137 kDa, Complex)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (36 kDa, Protein from Homo sapiens)
- 2-({1-[2-(4-chlorophenyl)-2-methylpropanoyl]piperidin-4-yl}amino)-5,6,7,8-tetrahydroquinazolin-4(3h)-one (428 Da, Ligand)
CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9dqj
Q-score: 0.095
Wang C, Liu Y, Lanier M, Yeager A, Singh I, Gumpper RH, Krumm BE, DeLeon C, Zhang S, Boehm M, Pittner R, Baron A, Dvorak L, Bacon C, Shoichet BK, Martinborough E, Fay JF, Cao C, Roth BL
Cell Rep (2024) 43 pp. 114942-114942 [ DOI: doi:10.1016/j.celrep.2024.114942 Pubmed: 39580805 ]
- G(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- 2-({1-[1-(4-methoxyphenyl)cyclopropane-1-carbonyl]piperidin-4-yl}amino)quinazolin-4(3h)-one (418 Da, Ligand)
- Ep-3945-bound mrgprd-gq complex (137 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- G(i) subunit alpha-2/g(s) subunit alpha isoforms xlas (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-2,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (28 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (36 kDa, Protein from Homo sapiens)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
Structure of Src in complex with beta-arrestin 1 revealing SH3 binding sites
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9bt8
Q-score: 0.539
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Proto-oncogene tyrosine-protein kinase src (54 kDa, Protein from Gallus gallus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Bacteriophage sp.)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Bacteriophage sp.)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with three-domain src (sh3-sh2-sh1). (Complex from Rattus norvegicus)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9cx3
Q-score: 0.423
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cx9
Q-score: 0.47
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8k4o
Q-score: 0.536
Liu A, Liu Y, Llinas Del Torrent Masachs C, Zhang W, Pardo L, Ye RD
PNAS (2024) 121 pp. e2403217121-e2403217121 [ DOI: doi:10.1073/pnas.2403217121 Pubmed: 39378089 ]
- Guanine nucleotide-binding protein subunit gamma (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (39 kDa, Protein from Homo sapiens)
- Kaposi's sarcoma-associated herpesvirus-g protein-coupled receptor (kshv-gpcr)in complex with cxc chemokine cxcl1 and gi protein (Complex from Homo sapiens)
- Growth-regulated alpha protein (6 kDa, Protein from Homo sapiens)
- G protein-coupled receptor (37 kDa, Protein from Human gammaherpesvirus 8)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (36 kDa, Protein from Homo sapiens)
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8k4p
Q-score: 0.555
Liu A, Liu Y, Llinas Del Torrent Masachs C, Zhang W, Pardo L, Ye RD
PNAS (2024) 121 pp. e2403217121-e2403217121 [ DOI: doi:10.1073/pnas.2403217121 Pubmed: 39378089 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- An active kaposi's sarcoma-associated herpesvirus-g protein-coupled receptor (kshv-gpcr) in complex with gi protein (Complex from Human gammaherpesvirus 8)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunit gamma (6 kDa, Protein from Homo sapiens)
- G protein-coupled receptor (33 kDa, Protein from Human gammaherpesvirus 8)
Lysophosphatidylserine receptor GPR34-Gi complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8iz4
Q-score: 0.52
Gong W, Liu G, Li X, Wang Y, Zhang X
To Be Published
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- [(2~{r})-2-oxidanyl-3-phosphonooxy-propyl] octadecanoate (438 Da, Ligand)
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 34 (64 kDa, Protein from Homo sapiens)
- Lysops-gpr34-gi-scfv16 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR34 receptor in complex with the antagonist YL-365
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 8iyx
Q-score: 0.448
Xia A, Yong X, Zhang C, Lin G, Jia G, Zhao C, Wang X, Hao Y, Wang Y, Zhou P, Yang X, Deng Y, Wu C, Chen Y, Zhu J, Tang X, Liu J, Zhang S, Zhang J, Xu Z, Hu Q, Zhao J, Yue Y, Yan W, Su Z, Wei Y, Zhou R, Dong H, Shao Z, Yang S
PNAS (2023) 120 pp. e2308435120-e2308435120 [ Pubmed: 37733739 DOI: doi:10.1073/pnas.2308435120 ]
- Cryo-em structure of the gpr34 receptor in complex with the antagonist yl-365 (Complex from Homo sapiens)
- 1-[4-(3-chlorophenyl)phenyl]carbonyl-4-[2-(4-phenylmethoxyphenyl)ethanoylamino]piperidine-4-carboxylic acid (583 Da, Ligand)
- Probable g-protein coupled receptor 34,probable g-protein coupled receptor 34,yl-365 (66 kDa, Protein from Homo sapiens)
GPR3 Orphan G-coupled Protein Receptor in complex with Dominant Negative Gs.
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8u8f
Q-score: 0.422
Russell IC, Zhang X, Bumbak F, McNeill SM, Josephs TM, Leeming MG, Christopoulos G, Venugopal H, Flocco MM, Sexton PM, Wootten D, Belousoff MJ
Biochemistry (2024) 63 pp. 625-631 [ DOI: doi:10.1021/acs.biochem.3c00647 Pubmed: 38376112 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Active state, gpr3 complex with dominant negative g-alphas. (Complex from Homo sapiens)
- G-protein coupled receptor 3 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)