Native human GABAA receptor of beta2-alpha1-beta2-alpha3-gamma2 assembly
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 9ctp
Q-score: 0.391
Zhou J, Noviello CM, Teng J, Moore H, Lega B, Hibbs RE
Nature (2025) [ DOI: doi:10.1038/s41586-024-08454-1 Pubmed: 39843743 ]
- Gamma-aminobutyric acid receptor subunit gamma-2 (50 kDa, Protein from Homo sapiens)
- Igg2b fab_1f4 heavy chain (49 kDa, Protein from Mus musculus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-3 (52 kDa, Protein from Homo sapiens)
- Native gabaa receptor from human brain (Complex from Homo sapiens)
- Kappa fab_1f4 light chain (23 kDa, Protein from Mus musculus)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1 (48 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8g4x
Q-score: 0.504
Sun C, Zhu H, Clark S, Gouaux E
Nature (2023) 622 pp. 195-201 [ Pubmed: 37730991 DOI: doi:10.1038/s41586-023-06556-w ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Native gabaa receptor isolated from mouse brain containing one alpha1 subunit, in complex with 8e3-fab, gaba, and allopregnanolone (350 kDa, Complex from Mus musculus)
- Heavy chain of 8e3 fab (24 kDa, Protein from Mus musculus)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (55 kDa, Protein from Mus musculus)
- Allopregnanolone (318 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-3 (55 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-1 (51 kDa, Protein from Mus musculus)
- Light chain of 8e3 fab (23 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit beta-2 (59 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8g5f
Q-score: 0.491
Sun C, Zhu H, Clark S, Gouaux E
Nature (2023) 622 pp. 195-201 [ Pubmed: 37730991 DOI: doi:10.1038/s41586-023-06556-w ]
- Allopregnanolone (318 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Light chain of 8e3 fab (23 kDa, Protein from Mus musculus)
- Heavy chain of 8e3 fab (24 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit gamma-2 (55 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-3 (55 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-1 (51 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit beta-2 (59 kDa, Protein from Mus musculus)
- Native gabaa receptor isolated from mouse brain containing one alpha1 subunit, in complex with 8e3-fab, gaba, and allopregnanolone (350 kDa, Complex from Mus musculus)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8g5g
Q-score: 0.435
Sun C, Zhu H, Clark S, Gouaux E
Nature (2023) 622 pp. 195-201 [ Pubmed: 37730991 DOI: doi:10.1038/s41586-023-06556-w ]
- Allopregnanolone (318 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Native gabaa receptor isolated from mouse brain containing one alpha1 subunit, in complex with 8e3-fab, gaba, zolpidem, and endogenous neurosteroid (350 kDa, Complex from Mus musculus)
- Zolpidem (307 Da, Ligand)
- Light chain of 8e3 fab (23 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-3 (55 kDa, Protein from Mus musculus)
- Heavy chain of 8e3 fab (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (55 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-1 (51 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit beta-2 (59 kDa, Protein from Mus musculus)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8g5h
Q-score: 0.477
Sun C, Zhu H, Clark S, Gouaux E
Nature (2023) 622 pp. 195-201 [ Pubmed: 37730991 DOI: doi:10.1038/s41586-023-06556-w ]
- Gamma-amino-butanoic acid (103 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Allopregnanolone (318 Da, Ligand)
- Native gabaa receptor isolated from mouse brain containing one alpha1 subunit, in complex with 8e3-fab, gaba, zolpidem, and endogenous neurosteroid (350 kDa, Complex from Mus musculus)
- Zolpidem (307 Da, Ligand)
- Light chain of 8e3 fab (23 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-3 (55 kDa, Protein from Mus musculus)
- Heavy chain of 8e3 fab (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (55 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit alpha-1 (51 kDa, Protein from Mus musculus)
- Gamma-aminobutyric acid receptor subunit beta-2 (59 kDa, Protein from Mus musculus)