Structure of the human Mediator-bound transcription pre-initiation complex
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7lbm
Q-score: 0.074
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 23 (156 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Super core promoter sense strand (31 kDa, DNA from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 8 (29 kDa, Protein from Homo sapiens)
- Super core promoter antisense strand (30 kDa, DNA from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 6 (28 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 22 (22 kDa, Protein from Homo sapiens)
- Cyclin-dependent kinase 7 (39 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 15 (86 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 28 (19 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 27 (35 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 19 (26 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Cyclin-h (37 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 30 (20 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 25 (78 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 7 (27 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 17 (72 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 21 (15 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Cdk-activating kinase assembly factor mat1 (35 kDa, Protein from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 14 (160 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Promoter dna (Complex from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 4 (29 kDa, Protein from Homo sapiens)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4, p52 (52 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 29 (21 kDa, Protein from Homo sapiens)
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 10 (15 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Isoform 2 of mediator of rna polymerase ii transcription subunit 16 (93 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 1 (62 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 9 (16 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 24 (110 kDa, Protein from Homo sapiens)
- Mediator of rna polymerase ii transcription subunit 18 (23 kDa, Protein from Homo sapiens)
- Cyclin-h and associated factors (Complex from Homo sapiens)
Focused refinement on the cPIC within the human Mediator-bound transcription pre-initiation complex
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Focused refinement of cTFIIH within the human Mediator-bound transcription pre-initiation complex
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Focused refinement on Med1 within the human Mediator-bound transcription pre-initiation complex
Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y
Science (2021) 372 pp. 52-56 [ DOI: doi:10.1126/science.abg3074 Pubmed: 33707221 ]
- Mediator-bound pre-initiation complex (2 MDa, Complex)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Mediator (1 MDa, Complex from Homo sapiens)
- Promoter dna (Complex from Homo sapiens)
- Tfiih (500 kDa, Complex from Homo sapiens)
Human holo-PIC in the open state
Resolution: 8.6 Å
EM Method: Single-particle
Fitted PDBs: 5iy7
Q-score: 0.105
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Scp-y (23 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Scp-x (23 kDa, DNA from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Transcription elongation factor tfiis (34 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Human holo-pic in the open state (1 MDa, Complex)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- General transcription factors (820 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human holo-PIC in the initial transcribing state
Resolution: 7.9 Å
EM Method: Single-particle
Fitted PDBs: 5iy8
Q-score: 0.111
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Scp-y (25 kDa, DNA from Homo sapiens)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- Human holo-pic in the initial transcribing state (1 MDa, Complex)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- General transcription factors (820 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Scp-x (25 kDa, DNA from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)