To Be Published
Carazolol-activated human beta3 adrenergic receptor
Zheng S, Zhang S, Dai S, Chen K, Gao K, Sun X, Lin B, Liu X
Chempluschem (2024) 89 pp. e202400288-e202400288 [ DOI: doi:10.1002/cplu.202400288 Pubmed: 39046191 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Bos taurus)
- Camelid antibody vhh fragment (13 kDa, Protein from Lama glama)
- (2s)-1-(9h-carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol (298 Da, Ligand)
- Carazolol bounded human beta3 adrenergic receptor-gs protein complex with gbeta, ggamma, nb35 and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Single-chain fv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
- Beta-3 adrenergic receptor (34 kDa, Protein from Homo sapiens)
Epinephrine-activated human beta3 adrenergic receptor
Zheng S, Zhang S, Dai S, Chen K, Gao K, Sun X, Lin B, Liu X
Chempluschem (2024) 89 pp. e202400288-e202400288 [ DOI: doi:10.1002/cplu.202400288 Pubmed: 39046191 ]
- Epinephrine bounded human beta3 adrenergic receptor-gs protein complex with gbeta, ggamma, nb35 and scfv16 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Bos taurus)
- Beta-3 adrenergic receptor (34 kDa, Protein from Homo sapiens)
- Camelid antibody vhh fragment (13 kDa, Protein from Lama glama)
- L-epinephrine (183 Da, Ligand)
- Single-chain fv16 (26 kDa, Protein from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ DOI: doi:10.1371/journal.pbio.3002673 Pubmed: 39083706 ]
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Amino group (16 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ DOI: doi:10.1371/journal.pbio.3002673 Pubmed: 39083706 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
Cryo-EM Structure of GPR61-G protein complex stabilized by scFv16
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 8tb0
Q-score: 0.438
Lees JA, Dias JM, Rajamohan F, Fortin JP, O'Connor R, Kong JX, Hughes EAG, Fisher EL, Tuttle JB, Lovett G, Kormos BL, Unwalla RJ, Zhang L, Dechert Schmitt AM, Zhou D, Moran M, Stevens KA, Fennell KF, Varghese AE, Maxwell A, Cote EE, Zhang Y, Han S
Nat Commun (2023) 14 pp. 5938-5938 [ Pubmed: 37741852 DOI: doi:10.1038/s41467-023-41646-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpr61 fused to dominant negative g alpha s/i n18 chimera (111 kDa, Protein from Homo sapiens)
- Single-chain fv16 (28 kDa, Protein from Homo sapiens)
- Gpr61-galphas-gbeta1-ggamma2 complex with single-chain fv16 (Complex from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ Pubmed: 37673901 DOI: doi:10.1038/s41467-023-41199-5 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trk
Q-score: 0.548
eLife (2023) [ ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- M4 machr bound to agonist iperoxo in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7trp
Q-score: 0.561
eLife (2023) [ ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- M4 machr bound to agonist iperoxo and the pam ly2033298 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-6-methoxy-4-methylthieno[2,3-b]pyridine-2-carboxamide (311 Da, Ligand)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7trq
Q-score: 0.587
eLife (2023) [ ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist iperoxo and the pam vu0467154 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- 5-amino-3,4-dimethyl-n-{[4-(trifluoromethanesulfonyl)phenyl]methyl}thieno[2,3-c]pyridazine-6-carboxamide (444 Da, Ligand)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)