Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ Pubmed: 37673901 DOI: doi:10.1038/s41467-023-41199-5 ]
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trk
Q-score: 0.548
eLife (2023) [ ]
- M4 machr bound to agonist iperoxo in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7trp
Q-score: 0.561
eLife (2023) [ ]
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-6-methoxy-4-methylthieno[2,3-b]pyridine-2-carboxamide (311 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist iperoxo and the pam ly2033298 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7trq
Q-score: 0.587
eLife (2023) [ ]
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- 5-amino-3,4-dimethyl-n-{[4-(trifluoromethanesulfonyl)phenyl]methyl}thieno[2,3-c]pyridazine-6-carboxamide (444 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist iperoxo and the pam vu0467154 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the endogenous agonist acetylcholine
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trs
Q-score: 0.503
eLife (2023) [ ]
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist acetylcholine in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)