Cryo-EM Structure of GPR61-G protein complex stabilized by scFv16
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 8tb0
Q-score: 0.438
Lees JA, Dias JM, Rajamohan F, Fortin JP, O'Connor R, Kong JX, Hughes EAG, Fisher EL, Tuttle JB, Lovett G, Kormos BL, Unwalla RJ, Zhang L, Dechert Schmitt AM, Zhou D, Moran M, Stevens KA, Fennell KF, Varghese AE, Maxwell A, Cote EE, Zhang Y, Han S
Nat Commun (2023) 14 pp. 5938-5938 [ DOI: doi:10.1038/s41467-023-41646-3 Pubmed: 37741852 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpr61 fused to dominant negative g alpha s/i n18 chimera (111 kDa, Protein from Homo sapiens)
- Single-chain fv16 (28 kDa, Protein from Homo sapiens)
- Gpr61-galphas-gbeta1-ggamma2 complex with single-chain fv16 (Complex from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ Pubmed: 37673901 DOI: doi:10.1038/s41467-023-41199-5 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7d7m
Q-score: 0.47
Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T
Structure (2021) 29 pp. 252-260.e6 [ Pubmed: 33264604 DOI: doi:10.1016/j.str.2020.11.007 ]
- Nanobody nb35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype,guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pge2-ep4-gs-gbeta1-ggamma2-nb35 complex (120 kDa, Complex)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Nanobody nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype,prostaglandin e2 receptor ep4 subtype (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)