Epinephrine-activated human beta3 adrenergic receptor
Zheng S, Zhang S, Dai S, Chen K, Gao K, Sun X, Lin B, Liu X
Chempluschem (2024) 89 pp. e202400288-e202400288 [ Pubmed: 39046191 DOI: doi:10.1002/cplu.202400288 ]
- Water (18 Da, Ligand)
- Single-chain fv16 (26 kDa, Protein from Homo sapiens)
- Beta-3 adrenergic receptor (34 kDa, Protein from Homo sapiens)
- Camelid antibody vhh fragment (13 kDa, Protein from Lama glama)
- Epinephrine bounded human beta3 adrenergic receptor-gs protein complex with gbeta, ggamma, nb35 and scfv16 (Complex from Homo sapiens)
- L-epinephrine (183 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Bos taurus)
Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Amino group (16 Da, Ligand)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ Pubmed: 37673901 DOI: doi:10.1038/s41467-023-41199-5 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the endogenous agonist acetylcholine
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trs
Q-score: 0.503
eLife (2023) [ ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist acetylcholine in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16. (Complex from Homo sapiens)
Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7d7m
Q-score: 0.47
Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T
Structure (2021) 29 pp. 252-260.e6 [ Pubmed: 33264604 DOI: doi:10.1016/j.str.2020.11.007 ]
- Nanobody nb35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype,guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pge2-ep4-gs-gbeta1-ggamma2-nb35 complex (120 kDa, Complex)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Nanobody nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype,prostaglandin e2 receptor ep4 subtype (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)