Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7lll
Q-score: 0.479
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ Pubmed: 35013280 DOI: doi:10.1038/s41467-021-27760-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Exendin-4 (4 kDa, Protein from Heloderma suspectum)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Exendin-4-glp-1r-gs complex (Complex from Homo sapiens)
Oxyntomodulin-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lly
Q-score: 0.452
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ Pubmed: 35013280 DOI: doi:10.1038/s41467-021-27760-0 ]
- Oxyntomodulin-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Pro-glucagon (4 kDa, Protein from Sus scrofa)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Ex4-D-Ala bound to the glucagon-like peptide-1 receptor/g protein complex (conformer 1)
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 7s1m
Q-score: 0.581
Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH
Nat Chem Biol (2022) 18 pp. 256-263 [ DOI: doi:10.1038/s41589-021-00945-w Pubmed: 34937906 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Ex4-d-ala (4 kDa, Protein from synthetic construct)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Glp-1r bound to ex4-d-ala, g alpha s, g beta-1, g gamma-2, and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Ex4-D-Ala bound to the glucagon-like peptide-1 receptor/g protein complex (conformer 2)
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7s3i
Q-score: 0.58
Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH
Nat Chem Biol (2022) 18 pp. 256-263 [ DOI: doi:10.1038/s41589-021-00945-w Pubmed: 34937906 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Glp-1r bound to ex4-d-ala, g alpha s, g beta-1, g gamma-2, and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Semaglutide-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in Complex with Gs protein
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7ki0
Q-score: 0.557
Zhang X, Belousoff MJ, Liang YL, Danev R, Sexton PM, Wootten D
Cell Rep (2021) 36 pp. 109374-109374 [ DOI: doi:10.1016/j.celrep.2021.109374 Pubmed: 34260945 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Semaglutide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Semaglutide-glp-1r-gs complex (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- 17-amino-10-oxo-3,6,12,15-tetraoxa-9-azaheptadecan-1-oic acid (308 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Taspoglutide-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in Complex with Gs Protein
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7ki1
Q-score: 0.535
Zhang X, Belousoff MJ, Liang YL, Danev R, Sexton PM, Wootten D
Cell Rep (2021) 36 pp. 109374-109374 [ DOI: doi:10.1016/j.celrep.2021.109374 Pubmed: 34260945 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Taspoglutide (3 kDa, Protein from synthetic construct)
- Taspoglutide-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R):Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lci
Q-score: 0.428
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ DOI: doi:10.1016/j.str.2021.04.008 Pubmed: 33957078 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Glp-1:gs complex with small molecule agonist bound (pf-06882961) (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R):Gs complex
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7lcj
Q-score: 0.533
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ DOI: doi:10.1016/j.str.2021.04.008 Pubmed: 33957078 ]
- Water (18 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Receptor only portion of glp1:gs complex with a small molecule agonist bound: pf-06882961 (Complex from Homo sapiens)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R)
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7lck
Q-score: 0.477
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ DOI: doi:10.1016/j.str.2021.04.008 Pubmed: 33957078 ]
- Water (18 Da, Ligand)
- Glp-1:gs complex with small molecule agonist (pf-06882961) bound (Complex from Homo sapiens)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 6x18
Q-score: 0.563
Zhang X, Belousoff MJ, Zhao P, Kooistra AJ, Truong TT, Ang SY, Underwood CR, Egebjerg T, Senel P, Stewart GD, Liang YL, Glukhova A, Venugopal H, Christopoulos A, Furness SGB, Miller LJ, Reedtz-Runge S, Langmead CJ, Gloriam DE, Danev R, Sexton PM, Wootten D
Mol Cell (2020) 80 pp. 485- [ Pubmed: 33027691 DOI: doi:10.1016/j.molcel.2020.09.020 ]
- Glp-1 peptide hormone bound to glucagon-like peptide-1 (glp-1) receptor (120 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)