Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7lll
Q-score: 0.479
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ DOI: doi:10.1038/s41467-021-27760-0 Pubmed: 35013280 ]
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Exendin-4 (4 kDa, Protein from Heloderma suspectum)
- Exendin-4-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Oxyntomodulin-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lly
Q-score: 0.452
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ DOI: doi:10.1038/s41467-021-27760-0 Pubmed: 35013280 ]
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Oxyntomodulin-glp-1r-gs complex (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pro-glucagon (4 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Ex4-D-Ala bound to the glucagon-like peptide-1 receptor/g protein complex (conformer 1)
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 7s1m
Q-score: 0.581
Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH
Nat Chem Biol (2022) 18 pp. 256-263 [ DOI: doi:10.1038/s41589-021-00945-w Pubmed: 34937906 ]
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Ex4-d-ala (4 kDa, Protein from synthetic construct)
- Glp-1r bound to ex4-d-ala, g alpha s, g beta-1, g gamma-2, and nb35 (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Ex4-D-Ala bound to the glucagon-like peptide-1 receptor/g protein complex (conformer 2)
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7s3i
Q-score: 0.58
Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH
Nat Chem Biol (2022) 18 pp. 256-263 [ DOI: doi:10.1038/s41589-021-00945-w Pubmed: 34937906 ]
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Glp-1r bound to ex4-d-ala, g alpha s, g beta-1, g gamma-2, and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
CryoEM Structure of the glucagon receptor with a dual-agonist peptide
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6whc
Q-score: 0.445
Chang R, Zhang X, Qiao A, Dai A, Belousoff MJ, Tan Q, Shao L, Zhong L, Lin G, Liang YL, Ma L, Han S, Yang D, Danev R, Wang MW, Wootten D, Wu B, Sexton PM
J Biol Chem (2020) 295 pp. 9313-9325 [ DOI: doi:10.1074/jbc.RA120.013793 Pubmed: 32371397 ]
- Nanobody 35 (Complex from Lama glama)
- Glucagon receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Dual-agonist peptide (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glucagon receptor complex (Complex)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor, glucagon receptor (Complex from Homo sapiens)
- Dual-agonist peptide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Non-peptide agonist (TT-OAD2) bound to the Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6orv
Q-score: 0.538
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ Pubmed: 31915381 DOI: doi:10.1038/s41586-019-1902-z ]
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Tt-oad2:glp-1r:gs complex (120 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- N-{(3s,8s)-3-{4-[(3,4-dichlorophenyl)methoxy]phenyl}-7-[(1s)-1-phenylpropyl]-2,3,6,7,8,9-hexahydro[1,4]dioxino[2,3-g]isoquinoline-8-carbonyl}-4-(2,3-dimethylpyridin-4-yl)-l-phenylalanine (856 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)