Cryo-EM structure of the GLP-1R/GCGR dual agonist Peptide 15-bound human GCGR-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8jiq
Q-score: 0.437
Li Y, Zhou Q, Dai A, Zhao F, Chang R, Ying T, Wu B, Yang D, Wang MW, Cong Z
PNAS (2023) 120 pp. e2303696120-e2303696120 [ DOI: doi:10.1073/pnas.2303696120 Pubmed: 37549266 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Peptide 15 (3 kDa, Protein from synthetic construct)
- Glucagon receptor (46 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glp-1r/gcgr dual agonist peptide 15-bound human gcgr-gs complex (Complex from Homo sapiens)
Cryo-EM structure of the WB4-24-bound hGLP-1R-Gs complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7x8s
Q-score: 0.482
Cong Z, Zhou Q, Li Y, Chen LN, Zhang ZC, Liang A, Liu Q, Wu X, Dai A, Xia T, Wu W, Zhang Y, Yang D, Wang MW
PNAS (2022) 119 pp. e2200155119-e2200155119 [ Pubmed: 35561211 DOI: doi:10.1073/pnas.2200155119 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-(2-methylpropanoylamino)phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid (1 kDa, Ligand)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with wb4-24 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbh
Q-score: 0.397
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ DOI: doi:10.1038/s41467-022-28683-0 Pubmed: 35217653 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Cryo-em structure of the peptide 20-bound human glucagon-like peptide-1 receptor in complex with gs protein (Complex from Homo sapiens)
Cryo-EM structure of the tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fim
Q-score: 0.409
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ DOI: doi:10.1038/s41467-022-28683-0 Pubmed: 35217653 ]
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with tirzepatide and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,human glucagon like peptide 1 receptor (68 kDa, Protein from Homo sapiens)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GCGR-Gs complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7v35
Q-score: 0.373
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ DOI: doi:10.1038/s41467-022-28683-0 Pubmed: 35217653 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the gipr/glp-1r/gcgr triagonist peptide 20-bound human gcgr-gs complex (Complex from Homo sapiens)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Glucagon receptor (46 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Peptide 20 (4 kDa, Protein from synthetic construct)
Cryo-EM structure of the non-acylated tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbi
Q-score: 0.533
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ DOI: doi:10.1038/s41467-022-28683-0 Pubmed: 35217653 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Non-acylated_tirzepatide (3 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with non-acylated_tirzepatide and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Isoform 3 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
Cryo-EM structure of the compound 2-bound human GLP-1 receptor-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7dur
Q-score: 0.273
Cong Z, Chen LN, Ma H, Zhou Q, Zou X, Ye C, Dai A, Liu Q, Huang W, Sun X, Wang X, Xu P, Zhao L, Xia T, Zhong W, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2021) 12 pp. 3763-3763 [ Pubmed: 34145245 DOI: doi:10.1038/s41467-021-24058-z ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- N-tert-butyl-6,7-bis(chloranyl)quinoxalin-2-amine (270 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein (Complex from Homo sapiens)
- Cryo-em structure of the compound 2-bound human glp-1 receptor-gs complex (Complex)
- Nanobody-35 (Complex from synthetic construct)
- Cholesterol (386 Da, Ligand)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
CryoEM Structure of the glucagon receptor with a dual-agonist peptide
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6whc
Q-score: 0.445
Chang R, Zhang X, Qiao A, Dai A, Belousoff MJ, Tan Q, Shao L, Zhong L, Lin G, Liang YL, Ma L, Han S, Yang D, Danev R, Wang MW, Wootten D, Wu B, Sexton PM
J Biol Chem (2020) 295 pp. 9313-9325 [ Pubmed: 32371397 DOI: doi:10.1074/jbc.RA120.013793 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Glucagon receptor (54 kDa, Protein from Homo sapiens)
- Glucagon receptor complex (Complex)
- Dual-agonist peptide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor, glucagon receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Dual-agonist peptide (Complex from synthetic construct)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
Cryo-EM structure of the human glucagon receptor in complex with Gs
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6lmk
Q-score: 0.443
Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B
Science (2020) 367 pp. 1346-1352 [ Pubmed: 32193322 DOI: doi:10.1126/science.aaz5346 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Complex of glucagon receptor bound to glucagon, gs protein and nanobody (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glucagon receptor (48 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human glucagon receptor in complex with Gi1
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6lml
Q-score: 0.425
Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B
Science (2020) 367 pp. 1346-1352 [ Pubmed: 32193322 DOI: doi:10.1126/science.aaz5346 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Glucagon receptor (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Complex of glucagon receptor bound to glucagon, gi1 protein and antibody (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)