Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8jru
Q-score: 0.438
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to beta-arrestin 1 (Complex from Homo sapiens)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Bos taurus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jrv
Q-score: 0.431
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to glucagon and beta-arrestin 1 (Complex from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Homo sapiens)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7xm8
Q-score: 0.361
To Be Published
- Glucagon (3 kDa, Protein from Homo sapiens)
- Glucagon amyloid fibril (Complex from Homo sapiens)
Cryo-EM structure of the compound 2-bound human GLP-1 receptor-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7dur
Q-score: 0.273
Cong Z, Chen LN, Ma H, Zhou Q, Zou X, Ye C, Dai A, Liu Q, Huang W, Sun X, Wang X, Xu P, Zhao L, Xia T, Zhong W, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2021) 12 pp. 3763-3763 [ Pubmed: 34145245 DOI: doi:10.1038/s41467-021-24058-z ]
- Guanine nucleotide-binding protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the compound 2-bound human glp-1 receptor-gs complex (Complex)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from synthetic construct)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- N-tert-butyl-6,7-bis(chloranyl)quinoxalin-2-amine (270 Da, Ligand)
- Glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
Cryo-EM structure of the human glucagon-like peptide-2 receptor-Gs protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7d68
Q-score: 0.524
Sun W, Chen LN, Zhou Q, Zhao LH, Yang D, Zhang H, Cong Z, Shen DD, Zhao F, Zhou F, Cai X, Chen Y, Zhou Y, Gadgaard S, van der Velden WJC, Zhao S, Jiang Y, Rosenkilde MM, Xu HE, Zhang Y, Wang MW
Cell Res (2020) 30 pp. 1098-1108 [ Pubmed: 33239759 DOI: doi:10.1038/s41422-020-00442-0 ]
- Guanine nucleotide-binding protein (Complex from Bos taurus)
- Water (18 Da, Ligand)
- Glucagon-like peptide 2 receptor (75 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Bos taurus)
- Pro-glucagon (3 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from synthetic construct)
- Cryo-em structure of the human glucagon-like peptide-2 receptor-gs protein complex (Complex)
- Glucagon-like peptide 2 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6xox
Q-score: 0.482
Kawai T, Sun B, Yoshino H, Feng D, Suzuki Y, Fukazawa M, Nagao S, Wainscott DB, Showalter AD, Droz BA, Kobilka TS, Coghlan MP, Willard FS, Kawabe Y, Kobilka BK, Sloop KW
PNAS (2020) 117 pp. 29959-29967 [ DOI: doi:10.1073/pnas.2014879117 Pubmed: 33177239 ]
- Nanobody 35 (Complex from Lama glama)
- Single-chain variable fragment 16 (Complex from Mus musculus)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glucagon-like peptide-1 receptor in complex with g protein and a non-peptide agonist ly3502970 (150 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Alpha subunit of gs with n-terminus swapped with equivalent residues in gi,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
- 3-[(1s,2s)-1-(5-[(4s)-2,2-dimethyloxan-4-yl]-2-{(4s)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1h-indazol-5-yl)-2-oxo-2,3-dihydro-1h-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5h-pyrazolo[4,3-c]pyridine-5-carbonyl}-1h-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4h)-one (882 Da, Ligand)