Cryo-EM structure of the GLP-1(9-36)-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9ivg
Q-score: 0.48
Li J, Li G, Mai Y, Liu X, Yang D, Zhou Q, Wang MW
Acta Pharm Sin B (2024) 14 pp. 5069-5073 [ Pubmed: 39664415 DOI: doi:10.1016/j.apsb.2024.09.002 ]
- Glp-1(9-36) (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glp-1(9-36)-bound human glp-1r-gs complex (Complex from Homo sapiens)
Cryo-EM structure of the GLP-1(9-36)-bound human GLP-1R-Gs complex in the presence of LSN3318839
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 9ivm
Q-score: 0.455
Li J, Li G, Mai Y, Liu X, Yang D, Zhou Q, Wang MW
Acta Pharm Sin B (2024) 14 pp. 5069-5073 [ Pubmed: 39664415 DOI: doi:10.1016/j.apsb.2024.09.002 ]
- Cryo-em structure of the glp-1(9-36)-bound human glp-1r-gs complex in the presence of lsn3318839 (Complex from Homo sapiens)
- Glp-1(9-36) (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- (2~{r})-2-[2-[1-[(1~{r})-1-[2,6-bis(chloranyl)-3-cyclopropyl-phenyl]ethyl]imidazo[4,5-c]pyridin-6-yl]phenyl]propanoic acid (480 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GLP-1R/GCGR dual agonist Peptide 15-bound human GCGR-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8jiq
Q-score: 0.437
Li Y, Zhou Q, Dai A, Zhao F, Chang R, Ying T, Wu B, Yang D, Wang MW, Cong Z
PNAS (2023) 120 pp. e2303696120-e2303696120 [ DOI: doi:10.1073/pnas.2303696120 Pubmed: 37549266 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Peptide 15 (3 kDa, Protein from synthetic construct)
- Glucagon receptor (46 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glp-1r/gcgr dual agonist peptide 15-bound human gcgr-gs complex (Complex from Homo sapiens)
Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8jru
Q-score: 0.438
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ DOI: doi:10.1038/s41586-023-06420-x Pubmed: 37558880 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- The glucagon receptor bound to beta-arrestin 1 (Complex from Homo sapiens)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Bos taurus)
Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jrv
Q-score: 0.431
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ DOI: doi:10.1038/s41586-023-06420-x Pubmed: 37558880 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Homo sapiens)
- The glucagon receptor bound to glucagon and beta-arrestin 1 (Complex from Homo sapiens)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7xm8
Q-score: 0.361
To Be Published
- Glucagon (3 kDa, Protein from Homo sapiens)
- Glucagon amyloid fibril (Complex from Homo sapiens)
Cryo-EM structure of the WB4-24-bound hGLP-1R-Gs complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7x8s
Q-score: 0.482
Cong Z, Zhou Q, Li Y, Chen LN, Zhang ZC, Liang A, Liu Q, Wu X, Dai A, Xia T, Wu W, Zhang Y, Yang D, Wang MW
PNAS (2022) 119 pp. e2200155119-e2200155119 [ Pubmed: 35561211 DOI: doi:10.1073/pnas.2200155119 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-(2-methylpropanoylamino)phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid (1 kDa, Ligand)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with wb4-24 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
GLP-1 receptor bound with Pfizer small molecule agonist
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7s15
Q-score: 0.405
Griffith DA, Edmonds DJ, Fortin JP, Kalgutkar AS, Kuzmiski JB, Loria PM, Saxena AR, Bagley SW, Buckeridge C, Curto JM, Derksen DR, Dias JM, Griffor MC, Han S, Jackson VM, Landis MS, Lettiere D, Limberakis C, Liu Y, Mathiowetz AM, Patel JC, Piotrowski DW, Price DA, Ruggeri RB, Tess DA
J Med Chem (2022) 65 pp. 8208-8226 [ Pubmed: 35647711 DOI: doi:10.1021/acs.jmedchem.1c01856 ]
cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R in apo form
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rg9
Q-score: 0.476
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor in apo form (Complex from Homo sapiens)
- Cryo-em of apo form human glucagon-like peptide 1 receptor glp-1r, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbh
Q-score: 0.397
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ DOI: doi:10.1038/s41467-022-28683-0 Pubmed: 35217653 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Cryo-em structure of the peptide 20-bound human glucagon-like peptide-1 receptor in complex with gs protein (Complex from Homo sapiens)