CryoEM structure of Western equine encephalitis virus VLP in complex with the avian MXRA8 receptor
Resolution: 3.92 Å
EM Method: Single-particle
Fitted PDBs: 8ewf
Q-score: 0.321
Zimmerman O, Zimmerman MI, Nelson CA, Raju S, Errico JM, Madden EA, Hassan AO, VanBlargan LA, Kim AS, Adams LJ, Basore K, Whitener BM, Earnest JT, Holmes AC, Ebel GD, Zmasek C, Scheuermann RH, Fremont DH, Diamond MS
To Be Published
- Chimeric mxra8 receptor: d1 from duck mxra8 and d2 from mouse mxra8 (30 kDa, Protein from Anas platyrhynchos)
- E1 envelope protein (47 kDa, Protein from Chikungunya virus)
- Spike glycoprotein e2 (46 kDa, Protein from Chikungunya virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Chikungunya virus vlp in complex with a chimeric mxra8 receptor (avian d1 with mammalian d2) (495 MDa, Complex from Chikungunya virus)
Late pre-fusion state of EEEV (pH 5.5) with localized reconstruction
Resolution: 14.1 Å
EM Method: Single-particle
Fitted PDBs: 7n69
Q-score: 0.081
Chen CL, Klose T, Sun C, Kim AS, Buda G, Rossmann MG, Diamond MS, Klimstra WB, Kuhn RJ
PNAS (2022) 119 pp. e2114119119-e2114119119 [ DOI: doi:10.1073/pnas.2114119119 Pubmed: 35867819 ]
Early pre-fusion state of EEEV (pH 5.5) with localized reconstruction
Resolution: 14.3 Å
EM Method: Single-particle
Fitted PDBs: 7n6a
Q-score: 0.069
Chen CL, Klose T, Sun C, Kim AS, Buda G, Rossmann MG, Diamond MS, Klimstra WB, Kuhn RJ
PNAS (2022) 119 pp. e2114119119-e2114119119 [ DOI: doi:10.1073/pnas.2114119119 Pubmed: 35867819 ]
CryoEM structure of Venezuelan Equine Encephalitis virus (VEEV) TC-83 strain VLP
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7sfu
Q-score: 0.332
Kafai NM, Williamson LE, Binshtein E, Sukupolvi-Petty S, Gardner CL, Liu J, Mackin S, Kim AS, Kose N, Carnahan RH, Jung A, Droit L, Reed DS, Handley SA, Klimstra WB, Crowe JE, Diamond MS
J Exp Med (2022) 219 [ Pubmed: 35297953 DOI: doi:10.1084/jem.20212532 ]
- Spike glycoprotein e2 (47 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Venezuelan equine encephalitis virus (strain tc-83) (Virus from Venezuelan equine encephalitis virus (strain TC-83))
- Spike glycoprotein e1 (47 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Capsid protein (18 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7sfv
Q-score: 0.324
Kafai NM, Williamson LE, Binshtein E, Sukupolvi-Petty S, Gardner CL, Liu J, Mackin S, Kim AS, Kose N, Carnahan RH, Jung A, Droit L, Reed DS, Handley SA, Klimstra WB, Crowe JE, Diamond MS
J Exp Med (2022) 219 [ Pubmed: 35297953 DOI: doi:10.1084/jem.20212532 ]
- Spike glycoprotein e2 (46 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Capsid protein (17 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Venezuelan equine encephalitis virus (strain tc-83) (Virus from Venezuelan equine encephalitis virus (strain TC-83))
- Hveev-63 fab heavy chain (23 kDa, Protein from Homo sapiens)
- Hveev-63 fab light chain (22 kDa, Protein from Homo sapiens)
- Spike glycoprotein e1 (47 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7sfw
Q-score: 0.508
Kafai NM, Williamson LE, Binshtein E, Sukupolvi-Petty S, Gardner CL, Liu J, Mackin S, Kim AS, Kose N, Carnahan RH, Jung A, Droit L, Reed DS, Handley SA, Klimstra WB, Crowe JE, Diamond MS
J Exp Med (2022) 219 [ Pubmed: 35297953 DOI: doi:10.1084/jem.20212532 ]
- Spike glycoprotein e2 (47 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Hveev-63 fab light chain (11 kDa, Protein from Homo sapiens)
- Capsid protein (30 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- Venezuelan equine encephalitis virus (strain tc-83) (Virus from Venezuelan equine encephalitis virus (strain TC-83))
- Hveev-63 fab heavy chain (13 kDa, Protein from Homo sapiens)
- Spike glycoprotein e1 (47 kDa, Protein from Venezuelan equine encephalitis virus (strain TC-83))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Native Mayaro virus cryo EM map, resolution 4.8 Angstroms
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6w2u
Q-score: 0.439
Powell LA, Miller A, Fox JM, Kose N, Klose T, Kim AS, Bombardi R, Tennekoon RN, Dharshan de Silva A, Carnahan RH, Diamond MS, Rossmann MG, Kuhn RJ, Crowe Jr JE
Cell Host Microbe (2020) 28 pp. 699- [ DOI: doi:10.1016/j.chom.2020.07.008 Pubmed: 32783883 ]
- Spike glycoprotein e1 (41 kDa, Protein from Mayaro virus (strain Brazil))
- Native mayaro virus (Complex)
- Spike glycoprotein e2 (38 kDa, Protein from Mayaro virus (strain Brazil))
- Mayaro virus (Complex from Mayaro virus)