Identification, structure and agonist design of an androgen membrane receptor.
Yang Z, Ping YQ, Wang MW, Zhang C, Zhou SH, Xi YT, Zhu KK, Ding W, Zhang QY, Song ZC, Zhao RJ, He ZL, Wang MX, Qi L, Ullmann C, Ricken A, Schoneberg T, Gan ZJ, Yu X, Xiao P, Yi F, Liebscher I, Sun JP
Cell (2025) [ Pubmed: 39884271 DOI: doi:10.1016/j.cell.2025.01.006 ]
- 5-alpha-dihydrotestosterone (290 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Gs protein alpha subunit (41 kDa, Protein from Bos taurus)
- Complex of gpr133 with agonist and g protein trime (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Adhesion g-protein coupled receptor d1 (65 kDa, Protein from Homo sapiens)
Identification, structure and agonist design of an androgen membrane receptor
Yang Z, Ping YQ, Wang MW, Zhang C, Zhou SH, Xi YT, Zhu KK, Ding W, Zhang QY, Song ZC, Zhao RJ, He ZL, Wang MX, Qi L, Ullmann C, Ricken A, Schoneberg T, Gan ZJ, Yu X, Xiao P, Yi F, Liebscher I, Sun JP
Cell (2025) [ Pubmed: 39884271 DOI: doi:10.1016/j.cell.2025.01.006 ]
- Scfv16 (28 kDa, Protein from synthetic construct)
- Methyl 3-[2-(2,4-dimethylphenoxy)ethanoylamino]-4-(4-ethylpiperazin-1-yl)benzoate (425 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Gs protein alpha subunit (41 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Complex of gpr133 with agonist and g protein trimer (Complex from Bos taurus)
- Adhesion g-protein coupled receptor d1 (65 kDa, Protein from Homo sapiens)
Identification, structure and agonist design of an androgen membrane receptor
Yang Z, Ping YQ, Wang MW, Zhang C, Zhou SH, Xi YT, Zhu KK, Ding W, Zhang QY, Song ZC, Zhao RJ, He ZL, Wang MX, Qi L, Ullmann C, Ricken A, Schoneberg T, Gan ZJ, Yu X, Xiao P, Yi F, Liebscher I, Sun JP
Cell (2025) [ Pubmed: 39884271 DOI: doi:10.1016/j.cell.2025.01.006 ]
- (5s,8r,9s,10s,13s,14s,17s)-17-hydroxy-1,10,13-trimethyl-4,5,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one (302 Da, Ligand)
- Scfv16 (28 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Complex of gpr133 with agonist and g protein trimer. (Complex from Rattus norvegicus)
- Gs protein alpha subunit (41 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Adhesion g-protein coupled receptor d1 (65 kDa, Protein from Homo sapiens)
Identification, structure and agonist design of an androgen membrane receptor.
Yang Z, Ping YQ, Wang MW, Zhang C, Zhou SH, Xi YT, Zhu KK, Ding W, Zhang QY, Song ZC, Zhao RJ, He ZL, Wang MX, Qi L, Ullmann C, Ricken A, Schoneberg T, Gan ZJ, Yu X, Xiao P, Yi F, Liebscher I, Sun JP
Cell (2025) [ Pubmed: 39884271 DOI: doi:10.1016/j.cell.2025.01.006 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from synthetic construct)
- 5-alpha-dihydrotestosterone (290 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor d1 (34 kDa, Protein from Homo sapiens)
- Complex of gpr133 with agonist and g protein trime (Complex from Homo sapiens)
Identification, structure and agonist design of an androgen membrane receptor.
Yang Z, Ping YQ, Wang MW, Zhang C, Zhou SH, Xi YT, Zhu KK, Ding W, Zhang QY, Song ZC, Zhao RJ, He ZL, Wang MX, Qi L, Ullmann C, Ricken A, Schoneberg T, Gan ZJ, Yu X, Xiao P, Yi F, Liebscher I, Sun JP
Cell (2025) [ Pubmed: 39884271 DOI: doi:10.1016/j.cell.2025.01.006 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-alpha-dihydrotestosterone (290 Da, Ligand)
- Gs protein alpha subunit (41 kDa, Protein from Homo sapiens)
- Complex of gpr133 with agonist and g protein trime (Complex from Homo sapiens)
- Adhesion g-protein coupled receptor d1 (65 kDa, Protein from Homo sapiens)
Cryo-EM structure of CH848.d949.10.17.GS-DH270.UCA3.G57R
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 9aug
Q-score: 0.436
Swanson OM, Zhang QE, Van Itallie E, Tian M, Brown AR, Harris C, Kapingidza AB, Rhodes B, Smith LM, Venkatayogi S, Cronin K, Frazier M, Parks R, Bar M, Jiang C, Martin Beem JS, Cheng HL, Davis J, McGovern K, Newman A, Edwards RJ, Cain D, Alam SM, Wiehe K, Saunders KO, Acharya P, Alt F, Haynes BF, Azoitei ML
Sci Transl Med (2025) 17 pp. eadr2218-eadr2218 [ Pubmed: 39772772 DOI: doi:10.1126/scitranslmed.adr2218 ]
- Uca3.g57r light chain (22 kDa, Protein from Homo sapiens)
- Uca3.g57r heavy chain (25 kDa, Protein from Homo sapiens)
- Hiv-1 bg505 ds-sosip glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Hiv-1 bg505 ds-sosip glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cryo-em structure of ch848.d949.10.17.gs-dh270.uca3.g57r (Complex from Human immunodeficiency virus 1)
Cryo-EM structure of CH848.d949.10.17.GS-DH270.UCA3
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9auh
Q-score: 0.466
Swanson OM, Zhang QE, Van Itallie E, Tian M, Brown AR, Harris C, Kapingidza AB, Rhodes B, Smith LM, Venkatayogi S, Cronin K, Frazier M, Parks R, Bar M, Jiang C, Martin Beem JS, Cheng HL, Davis J, McGovern K, Newman A, Edwards RJ, Cain D, Alam SM, Wiehe K, Saunders KO, Acharya P, Alt F, Haynes BF, Azoitei ML
Sci Transl Med (2025) 17 pp. eadr2218-eadr2218 [ DOI: doi:10.1126/scitranslmed.adr2218 Pubmed: 39772772 ]
- Uca3 light chain (22 kDa, Protein from Homo sapiens)
- Uca3 heavy chain (24 kDa, Protein from Homo sapiens)
- Hiv-1 bg505 ds-sosip glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Hiv-1 bg505 ds-sosip glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Cryo-em structure of ch848.d949.10.17.gs-dh270.uca3 (Complex from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of CH848.d949.10.17.GS-DH270.UCA4
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 9aui
Q-score: 0.451
Swanson OM, Zhang QE, Van Itallie E, Tian M, Brown AR, Harris C, Kapingidza AB, Rhodes B, Smith LM, Venkatayogi S, Cronin K, Frazier M, Parks R, Bar M, Jiang C, Martin Beem JS, Cheng HL, Davis J, McGovern K, Newman A, Edwards RJ, Cain D, Alam SM, Wiehe K, Saunders KO, Acharya P, Alt F, Haynes BF, Azoitei ML
Sci Transl Med (2025) 17 pp. eadr2218-eadr2218 [ Pubmed: 39772772 DOI: doi:10.1126/scitranslmed.adr2218 ]
- Hiv-1 bg505 ds-sosip glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Cryo-em structure of ch848.d949.10.17.gs-dh270.uca4 (Complex from Human immunodeficiency virus 1)
- Hiv-1 bg505 ds-sosip glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Uca4 light chain (22 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Uca4 heavy chain (24 kDa, Protein from Homo sapiens)
Human proton sensing receptor GPR65 in complex with miniGs - focused map of 7TM
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2024) [ DOI: doi:10.1016/j.cell.2024.11.036 Pubmed: 39753132 ]
- Gpr65 receptor in complex with minigs (Complex)
- Nanobody 35 (Complex from Lama glama)
- Minigs (alpha and beta subunits) (Complex from Homo sapiens)
- Gpr65 receptor (Complex from Homo sapiens)
Human proton sensing receptor GPR65 in complex with miniGs
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671- [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Psychosine receptor (40 kDa, Protein from Homo sapiens)
- Complex of gpr65 bound to gs heterotrimer (Complex)