FSHR-Follicle stimulating hormone-compound 716340-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8i2g
Q-score: 0.363
Duan J, Xu P, Zhang H, Luan X, Yang J, He X, Mao C, Shen DD, Ji Y, Cheng X, Jiang H, Jiang Y, Zhang S, Zhang Y, Xu HE
Nat Commun (2023) 14 pp. 519-519 [ Pubmed: 36720854 DOI: doi:10.1038/s41467-023-36170-3 ]
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Myristic acid (228 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Chimera of guanine nucleotide-binding protein g(s) subunit alpha and guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Follitropin subunit beta (12 kDa, Protein from Homo sapiens)
- N-tert-butyl-n-ethyl-8-methoxy-9-propan-2-yloxy-1-thiophen-2-yl-5,6-dihydroimidazo[5,1-a]isoquinoline-3-carboxamide (467 Da, Ligand)
- Fshr-follicle stimulating hormone-compound 716340-gs complex (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Follicle-stimulating hormone receptor (76 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure of A2BR bound to synthetic agonists BAY 60-6583
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hdo
Q-score: 0.497
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- A2br-g protein-bay 60-6583 complex (160 kDa, Complex from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- 2-[6-azanyl-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]pyridin-2-yl]sulfanylethanamide (379 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure of A2BR bound to endogenous agonists adenosine
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8hdp
Q-score: 0.428
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- A2br-g protein-adenosine complex (160 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- Adenosine (267 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
TSHR-thyroid stimulating hormone-Gs-ML109 complex
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 7xw5
Q-score: 0.482
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022) 609 pp. 854-859 [ Pubmed: 35940204 DOI: doi:10.1038/s41586-022-05173-3 ]
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Thyrotropin subunit beta (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- ~{n}-[4-[[2-methoxy-5-[(2~{s})-5-oxidanyl-4-oxidanylidene-3-(phenylmethyl)-1,2-dihydroquinazolin-2-yl]phenyl]methoxy]phenyl]ethanamide (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Nanobody35 (14 kDa, Protein from Lama glama)
- Tshr-thyroid stimulating hormone-gs-ml109 complex (Complex from Homo)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xz5
Q-score: 0.514
Xu P, Huang S, Guo S, Yun Y, Cheng X, He X, Cai P, Lan Y, Zhou H, Jiang H, Jiang Y, Xie X, Xu HE
Nat Struct Mol Biol (2022) 29 pp. 863-870 [ DOI: doi:10.1038/s41594-022-00816-5 Pubmed: 35970999 ]
- Gpr119-gs (Complex from Homo sapiens)
- Gpr119-gs-nb35-lpc complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- (4r,7r,18e)-4,7-dihydroxy-n,n,n-trimethyl-10-oxo-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium 4-oxide (522 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor (53 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7xz6
Q-score: 0.552
Xu P, Huang S, Guo S, Yun Y, Cheng X, He X, Cai P, Lan Y, Zhou H, Jiang H, Jiang Y, Xie X, Xu HE
Nat Struct Mol Biol (2022) 29 pp. 863-870 [ DOI: doi:10.1038/s41594-022-00816-5 Pubmed: 35970999 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Propan-2-yl 4-[1-(2-fluoranyl-4-methylsulfonyl-phenyl)pyrazolo[3,4-d]pyrimidin-4-yl]oxypiperidine-1-carboxylate (477 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpr119-gs (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpr119-gs-nb35-apd668 complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor (53 kDa, Protein from Homo sapiens)
TSHR-Gs-M22 antibody-ML109 complex
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 7xw6
Q-score: 0.441
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022) 609 pp. 854-859 [ Pubmed: 35940204 DOI: doi:10.1038/s41586-022-05173-3 ]
- The complex was assembled by co-expression. (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Nanobody35 (14 kDa, Protein from Homo sapiens)
- M22 antibody light chain (11 kDa, Protein from Mus musculus)
- Palmitic acid (256 Da, Ligand)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- ~{n}-[4-[[2-methoxy-5-[(2~{s})-5-oxidanyl-4-oxidanylidene-3-(phenylmethyl)-1,2-dihydroquinazolin-2-yl]phenyl]methoxy]phenyl]ethanamide (523 Da, Ligand)
- M22 antibody heavy chain (13 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 7xw7
Q-score: 0.04
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022) 609 pp. 854-859 [ Pubmed: 35940204 DOI: doi:10.1038/s41586-022-05173-3 ]
- K1-70 scfv (24 kDa, Protein from Homo sapiens)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- Tshr-thyroid stimulating hormone-gs-ml109 complex (Complex from Homo)
Cryo-EM structure of the SV1-Gs complex.
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7v9l
Q-score: 0.505
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ Pubmed: 34599099 DOI: doi:10.1073/pnas.2106606118 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with g protein (Complex from Homo sapiens)
- Ghrh receptor splice variant 1,ghrh receptor splice variant 1,ghrh receptor splice variant 1,sv1 (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GHRH-bound human GHRHR splice variant 1 complex
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7v9m
Q-score: 0.431
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ Pubmed: 34599099 DOI: doi:10.1073/pnas.2106606118 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with ghrh and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Human growth hormone releasing hormone receptor splice variant 1(sv1) (57 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)