Cryo-EM structure of the amthamine-bound H2R-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8yut
Q-score: 0.529
Shen Q, Tang X, Wen X, Cheng S, Xiao P, Zang SK, Shen DD, Jiang L, Zheng Y, Zhang H, Xu H, Mao C, Zhang M, Hu W, Sun JP, Zhang Y, Chen Z
Adv Sci (2024) 11 pp. e2310120-e2310120 [ DOI: doi:10.1002/advs.202310120 Pubmed: 38647423 ]
- Amthamine-bound h2r-gs complex (Complex from Homo sapiens)
- 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine (157 Da, Ligand)
- Histamine h2 receptor (46 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
FSHR-Follicle stimulating hormone-compound 716340-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8i2g
Q-score: 0.363
Duan J, Xu P, Zhang H, Luan X, Yang J, He X, Mao C, Shen DD, Ji Y, Cheng X, Jiang H, Jiang Y, Zhang S, Zhang Y, Xu HE
Nat Commun (2023) 14 pp. 519-519 [ DOI: doi:10.1038/s41467-023-36170-3 Pubmed: 36720854 ]
- Follicle-stimulating hormone receptor (76 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Follitropin subunit beta (12 kDa, Protein from Homo sapiens)
- Fshr-follicle stimulating hormone-compound 716340-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- N-tert-butyl-n-ethyl-8-methoxy-9-propan-2-yloxy-1-thiophen-2-yl-5,6-dihydroimidazo[5,1-a]isoquinoline-3-carboxamide (467 Da, Ligand)
- Nb35 (13 kDa, Protein from Lama glama)
- Chimera of guanine nucleotide-binding protein g(s) subunit alpha and guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (30 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Myristic acid (228 Da, Ligand)
Structural insights into human brain gut peptide cholecystokinin receptors
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xou
Q-score: 0.434
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
Cell Discov (2022) 8 pp. 55-55 [ Pubmed: 35672283 DOI: doi:10.1038/s41421-022-00420-3 ]
- Cck-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Human brain gut peptide cholecystokinin receptors (Complex)
- Nanobody 35 (13 kDa, Protein from synthetic construct)
- Cholecystokinin receptor1 (Complex from Homo sapiens)
- Gs protein (Complex from Homo sapiens)
- Cck-8 (Complex)
Structural insights into human brain gut peptide cholecystokinin receptors
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7xov
Q-score: -0.079
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
Cell Discov (2022) 8 pp. 55-55 [ Pubmed: 35672283 DOI: doi:10.1038/s41421-022-00420-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Nanobody 35 (13 kDa, Protein from synthetic construct)
- Cholecystokinin receptor1 (Complex)
- Cholecystokinin receptor1 (Complex from Homo sapiens)
- 2-[2-[[4-(4-chloranyl-2,5-dimethoxy-phenyl)-5-(2-cyclohexylethyl)-1,3-thiazol-2-yl]carbamoyl]-5,7-dimethyl-indol-1-yl]ethanoic acid (610 Da, Ligand)
- Gs protein (Complex from Homo sapiens)
Structural insights into human brain gut peptide cholecystokinin receptors
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xow
Q-score: 0.499
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
Cell Discov (2022) 8 pp. 55-55 [ Pubmed: 35672283 DOI: doi:10.1038/s41421-022-00420-3 ]
- Scfc16 (Complex from Mus musculus)
- Gastrin (2 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor (Complex from Homo sapiens)
- Gastrin (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Gastrin/cholecystokinin type b receptor (48 kDa, Protein from Homo sapiens)
- Human brain gut peptide cholecystokinin receptors (Complex)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs protein (Complex from Homo sapiens)
Cryo-EM structure of the R399-bound GPBAR-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtq
Q-score: 0.456
Ma L, Yang F, Wu X, Mao C, Guo L, Miao T, Zang SK, Jiang X, Shen DD, Wei T, Zhou H, Wei Q, Li S, Shu Q, Feng S, Jiang C, Chu B, Du L, Sun JP, Yu X, Zhang Y, Zhang P
PNAS (2022) 119 pp. e2117054119-e2117054119 [ DOI: doi:10.1073/pnas.2117054119 Pubmed: 35858343 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- R399-bound gpbar-gs complex (Complex)
- [2-(2,5-dichloro-phenoxy)-pyridin-3-yl]-(3,4-dihydro-2h-quinolin-1-yl)-methanone (399 Da, Ligand)
- Nanobody-35 (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Afamelanotide (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f55
Q-score: 0.411
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Bremelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Complex of bremelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Bremelanotide (1 kDa, Protein from synthetic construct)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f58
Q-score: 0.516
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocrotin-4 receptor (Complex from Homo sapiens)
- Complex of thiq bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (Complex)
- (3r)-n-[(2r)-3-(4-chlorophenyl)-1-[4-cyclohexyl-4-(1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-1-oxidanylidene-propan-2-yl]-1,2,3,4-tetrahydroisoquinoline-3-carboxamide (589 Da, Ligand)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)