the human PTH1 receptor bound to an intracellular biased agonist
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8gw8
Q-score: 0.511
Kobayashi K, Kawakami K, Kusakizako T, Tomita A, Nishimura M, Sawada K, Okamoto HH, Hiratsuka S, Nakamura G, Kuwabara R, Noda H, Muramatsu H, Shimizu M, Taguchi T, Inoue A, Murata T, Nureki O
Nature (2023) 618 pp. 1085-1093 [ DOI: doi:10.1038/s41586-023-06169-3 Pubmed: 37286611 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Nanobody nb35 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- The human pth1 receptor bound to an intracellular biased agonist (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Rattus norvegicus)
- Pco-371 (635 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Bos taurus)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex from Homo sapiens)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpr174/gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
PTHrP-bound human PTH1R in complex with Gs
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vvj
Q-score: 0.518
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Pthrp (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (55 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex)
- Nanobody nb35 (Complex)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)
- Pthrp[1-36] (4 kDa, Protein from Homo sapiens)
PTH-bound human PTH1R in complex with Gs (class1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7vvk
Q-score: 0.444
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Parathyroid hormone (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex)
- Nanobody nb35 (Complex)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)
PTH-bound human PTH1R in complex with Gs (class2)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7vvl
Q-score: 0.54
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Parathyroid hormone (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex)
- Nanobody nb35 (Complex)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)
- Water (18 Da, Ligand)
PTH-bound human PTH1R in complex with Gs (class3)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vvm
Q-score: 0.454
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Parathyroid hormone (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex)
- Nanobody nb35 (Complex)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)
PTH-bound human PTH1R in complex with Gs (class4)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7vvn
Q-score: 0.405
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Nanobody nb35 (Complex)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex from Homo sapiens)
- Parathyroid hormone (Complex)
PTH-bound human PTH1R in complex with Gs (class5)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7vvo
Q-score: 0.359
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell (2022) 82 pp. 3468-3483.e5 [ Pubmed: 35932760 DOI: doi:10.1016/j.molcel.2022.07.003 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Nanobody nb35 (15 kDa, Protein from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from unidentified)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Rattus norvegicus)
- Pthrp (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (Complex)
- Nanobody nb35 (Complex)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Endogenous ligand-bound the human pth receptor in complex with gs (Complex from Homo sapiens)