Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8gy7
Q-score: 0.453
Luo P, Feng W, Ma S, Dai A, Wu K, Chen X, Yuan Q, Cai X, Yang D, Wang MW, Eric Xu H, Jiang Y
Cell Res (2023) 33 pp. 46-54 [ DOI: doi:10.1038/s41422-022-00751-6 Pubmed: 36588120 ]
- Mrap1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Acth (Complex from Homo sapiens)
- Corticotropin (2 kDa, Protein from synthetic construct)
- Acth-bound melanocortin-2 receptor in complex with mrap1 and gs protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Melanocortin-2 receptor accessory protein (20 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-3,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (41 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,adrenocorticotropic hormone receptor,melanocortin-2 receptor,adrenocorticotropic hormone receptor,adrenocorticotropic hormone receptor (67 kDa, Protein from Homo sapiens)
- Melanocortin-2 receptor (Complex from Homo sapiens)
Cryo-EM structure of alpha-MSH-bound melanocortin-1 receptor in complex with Gs protein and Nb35
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f4d
Q-score: 0.478
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Alpha-melanocyte-stimulating hormone (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Nanobody 35 (Complex from Homo sapiens)
- Cryo-em structure of alpha-msh-bound melanocortin-1 receptor in complex with gs protein and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Melanocortin-1 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f4f
Q-score: 0.509
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Melanocortin-1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein and scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Afamelanotide (Complex)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7f4h
Q-score: 0.52
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Scfv16 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Afamelanotide (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein, nb35 and scfv16 (Complex from Homo sapiens)
Cryo-EM structure of SHU9119-bound melanocortin-1 receptor in complex with Gs protein and Nb35
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f4i
Q-score: 0.474
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Cryo-em structure of shu9119-bound melanocortin-1 receptor in complex with gs protein and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex)
- Shu9119 (Complex)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Shu9119 (1 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbf
Q-score: 0.444
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Palmitic acid (256 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nbh
Q-score: 0.347
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Palmitic acid (256 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbi
Q-score: 0.285
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)