Cryo-EM structure of the SV1-Gs complex.
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7v9l
Q-score: 0.505
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2106606118 Pubmed: 34599099 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Ghrh receptor splice variant 1,ghrh receptor splice variant 1,ghrh receptor splice variant 1,sv1 (57 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GHRH-bound human GHRHR splice variant 1 complex
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7v9m
Q-score: 0.431
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2106606118 Pubmed: 34599099 ]
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with ghrh and g protein (Complex from Homo sapiens)
- Human growth hormone releasing hormone receptor splice variant 1(sv1) (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic gonadotropin-Gs complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7fig
Q-score: 0.377
Duan J, Xu P, Cheng X, Mao C, Croll T, He X, Shi J, Luan X, Yin W, You E, Liu Q, Zhang S, Jiang H, Zhang Y, Jiang Y, Xu HE
Nature (2021) 598 pp. 688-692 [ Pubmed: 34552239 DOI: doi:10.1038/s41586-021-03924-2 ]
- Camelid antibody vhh fragment nb35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Luteinizing hormone/choriogonadotropin receptor(s277i)-chorionic gonadotropin-gs complex (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gs protein (Complex from Bos taurus)
- Choriogonadotropin receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Antibody (Complex from Lama glama)
- Luteinizing hormone (Complex from Homo sapiens)
- Engineered guanine nucleotide-binding protein g(s) subunit alpha (41 kDa, Protein from Bos taurus)
- Glycoprotein hormones alpha chain (13 kDa, Protein from Homo sapiens)
- Lutropin-choriogonadotropic hormone receptor (78 kDa, Protein from Homo sapiens)
- Choriogonadotropin subunit beta 3 (17 kDa, Protein from Homo sapiens)
- Chorionic gonadotropin (Complex from Homo sapiens)
luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic gonadotropin-Gs-Org43553 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7fih
Q-score: 0.382
Duan J, Xu P, Cheng X, Mao C, Croll T, He X, Shi J, Luan X, Yin W, You E, Liu Q, Zhang S, Jiang H, Zhang Y, Jiang Y, Xu HE
Nature (2021) 598 pp. 688-692 [ Pubmed: 34552239 DOI: doi:10.1038/s41586-021-03924-2 ]
- 5-azanyl-n-tert-butyl-2-methylsulfanyl-4-[3-(2-morpholin-4-ylethanoylamino)phenyl]thieno[2,3-d]pyrimidine-6-carboxamide (514 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Luteinizing hormone/choriogonadotropin receptor(s277i)-chorionic gonadotropin-gs-org43553 complex (Complex)
- Engineered guanine nucleotide-binding protein g(s) subunit alpha (41 kDa, Protein from Bos taurus)
- Choriogonadotropin receptor (Complex from Homo sapiens)
- Glycoprotein hormones alpha chain (12 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Luteinizing hormone (Complex from Homo sapiens)
- Lutropin-choriogonadotropic hormone receptor (78 kDa, Protein from Homo sapiens)
- Choriogonadotropin subunit beta 3 (17 kDa, Protein from Homo sapiens)
- Chorionic gonadotropin (Complex from Homo sapiens)
- Gs proteins (Complex from Bos taurus)
luteinizing hormone/choriogonadotropin receptor-chorionic gonadotropin-Gs complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7fii
Q-score: 0.326
Duan J, Xu P, Cheng X, Mao C, Croll T, He X, Shi J, Luan X, Yin W, You E, Liu Q, Zhang S, Jiang H, Zhang Y, Jiang Y, Xu HE
Nature (2021) 598 pp. 688-692 [ Pubmed: 34552239 DOI: doi:10.1038/s41586-021-03924-2 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Engineered guanine nucleotide-binding protein g(s) subunit alpha (41 kDa, Protein from Bos taurus)
- Lutropin-choriogonadotropic hormone receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Luteinizing hormone/choriogonadotropin receptor-chorionic gonadotropin-gs complex (Complex)
- Glycoprotein hormones alpha chain (13 kDa, Protein from Homo sapiens)
- Glycoprotein hormones alpha chain (Complex from Homo sapiens)
- Choriogonadotropin subunit beta 3 (17 kDa, Protein from Homo sapiens)
- Lutropin-choriogonadotropic hormone receptor (78 kDa, Protein from Homo sapiens)
- Choriogonadotropin subunit beta 3 (Complex from Homo sapiens)
- Gs proteins (Complex from Bos taurus)
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7cz5
Q-score: 0.582
Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2020) 11 pp. 5205-5205 [ Pubmed: 33060564 DOI: doi:10.1038/s41467-020-18945-0 ]
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Hormone receptor, g(s) subunit alpha isoforms (Complex from Homo sapiens)
- G(s) subunit gamma-2 (Complex from Bos taurus)
- Hormone (Complex from Homo sapiens)
- Nanobody35 (Complex from synthetic construct)
- G(s) subunit beta-1 (Complex from Rattus norvegicus)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor (61 kDa, Protein from Homo sapiens)
- Growth hormone-releasing hormone receptor - gs protein complex (180 kDa, Complex)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6pb0
Q-score: 0.455
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 1 (43 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Urocortin (4 kDa, Protein from Homo sapiens)
- Urocortin1-bound crf1r in complex gs and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6pb1
Q-score: 0.47
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Urocortin 1-bound corticotropin-releasing factor 2 receptor in complex with gs protein (Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Urocortin (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 2 (45 kDa, Protein from Homo sapiens)