Cryo-EM structure of the Methanosarcina mazei glutamine synthetase (GS) with Met-Sox-P and ADP
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8tfk
Q-score: 0.194
Schumacher MA, Salinas R, Travis BA, Singh RR, Lent N
Nat Commun (2023) 14 pp. 7375-7375 [ DOI: doi:10.1038/s41467-023-43243-w Pubmed: 37968329 ]
- M. mazei glutamine synthetase (gs) transition state complex (Complex from Methanosarcina mazei Go1)
- Magnesium ion (24 Da, Ligand)
- Glutamine synthetase (52 kDa, Protein from Methanosarcina mazei Go1)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- L-methionine-s-sulfoximine phosphate (260 Da, Ligand)
S. aureus GS(12)-Q-GlnR peptide
Resolution: 2.15 Å
EM Method: Single-particle
Fitted PDBs: 7tf6
Q-score: 0.697
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Dodecameric gs complex with glutamine and glnr c-tail peptides (Complex)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Peptide from glutamine synthetase repressor (1 kDa, Protein from Staphylococcus aureus)
- Glutamine synthetase (51 kDa, Protein from Staphylococcus aureus)
Resolution: 2.13 Å
EM Method: Single-particle
Fitted PDBs: 7tf7
Q-score: 0.695
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
L. monocytogenes GS(14)-Q-GlnR peptide
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 7tf9
Q-score: 0.598
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Tetradecameric l. monocytogenes gs complex with glutamine and glnr c-tail peptides (Complex)
- C-tail peptide of glutamine synthetase repressor (660 Da, Protein from Listeria monocytogenes)
- Glutamine synthetase (52 kDa, Protein from Listeria monocytogenes)
P. polymyxa GS(12)-Q-GlnR peptide
Resolution: 2.07 Å
EM Method: Single-particle
Fitted PDBs: 7tfa
Q-score: 0.677
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Glnr c-tail peptide (1 kDa, Protein from Paenibacillus polymyxa)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Glutamine synthetase (52 kDa, Protein from Paenibacillus polymyxa)
- Dodecameric p. polymyxa gs complex with glutamine and glnr c-tail peptides (Complex)
P. polymyxa GS(14)-Q-GlnR peptide
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 7tfb
Q-score: 0.649
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Glnr c-tail peptide (1 kDa, Protein from Paenibacillus polymyxa)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Tetradecameric p. polymyxa gs complex with glutamine and glnr c-tail peptides (Complex)
- Glutamine synthetase (52 kDa, Protein from Paenibacillus polymyxa)
B. subtilis GS(14)-Q-GlnR peptide
Resolution: 1.96 Å
EM Method: Single-particle
Fitted PDBs: 7tfc
Q-score: 0.701
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Magnesium ion (24 Da, Ligand)
- Glnr c-tail peptide (1 kDa, Protein from Bacillus subtilis)
- Glutamine synthetase (52 kDa, Protein from Bacillus subtilis)
- Glutamine (146 Da, Ligand)
- Tetradecameric b. subtilis gs complex with glutamine and glnr c-tail peptides (Complex)
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7tfd
Q-score: 0.437
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Magnesium ion (24 Da, Ligand)
- Apo dodecameric p. polymyxa gs complex (Complex from Paenibacillus polymyxa)
- Glutamine synthetase (52 kDa, Protein from Paenibacillus polymyxa)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7tfe
Q-score: 0.531
Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA
Nat Commun (2022) 13 pp. 3793-3793 [ Pubmed: 35778410 DOI: doi:10.1038/s41467-022-31573-0 ]
- Glutamine synthetase (52 kDa, Protein from Listeria monocytogenes)
- Magnesium ion (24 Da, Ligand)
- Apo dodecameric l. monocytogenes gs complex (Complex from Listeria monocytogenes)