cryo-EM structure of Gs-coupled zebrafish GPR4 at pH 6.5
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8zfz
Q-score: 0.41
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Zgpr4-gs complex (Complex from Danio rerio)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
cryo-EM structure of zebrafish GPR4-Gs complex at pH 8.5
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9j31
Q-score: 0.467
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Zgpr4-gs complex (Complex from Danio rerio)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Scfv16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pok
Q-score: 0.501
Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F
Nat Commun (2024) 15 pp. 1831-1831 [ Pubmed: 38418462 DOI: doi:10.1038/s41467-024-46096-z ]
- Histamine h2 receptor (41 kDa, Protein from Homo sapiens)
- Histamine h2 receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- H2 receptor coupled to heterotrimeric gs protein (Complex)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 and guanine nucleotide-binding protein subunit gamma (fragment) (Complex from Homo sapiens)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8ppu
Q-score: 0.532
Betancurt-Anzola L, Martinez-Carranza M, Delarue M, Zatopek KM, Gardner AF, Sauguet L
Nat Commun (2023) 14 pp. 8306-8306 [ DOI: doi:10.1038/s41467-023-44125-x Pubmed: 38097591 ]
- Magnesium ion (24 Da, Ligand)
- Dna polymerase ii small subunit (74 kDa, Protein from Pyrococcus abyssi GE5)
- Dna polymerase sliding clamp (29 kDa, Protein from Pyrococcus abyssi GE5)
- Binary complex of pold bound to pcna and a primer/template duplex containing three consecutive mismatches (Complex from Pyrococcus abyssi GE5)
- Dp2 (144 kDa, Protein from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- Dna (5'-d(p*ap*gp*cp*ap*cp*gp*gp*cp*tp*cp*gp*gp*cp*cp*cp*gp*g)-3') (7 kDa, DNA from Pyrococcus abyssi GE5)
- Dna (5'-d(p*cp*cp*gp*gp*gp*cp*cp*gp*ap*gp*cp*cp*gp*tp*(gs)p*(c7r)p*(pst)p*(pst)p*(pst))-3') (6 kDa, DNA from Pyrococcus abyssi GE5)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8ppv
Q-score: 0.547
Betancurt-Anzola L, Martinez-Carranza M, Delarue M, Zatopek KM, Gardner AF, Sauguet L
Nat Commun (2023) 14 pp. 8306-8306 [ DOI: doi:10.1038/s41467-023-44125-x Pubmed: 38097591 ]
- Magnesium ion (24 Da, Ligand)
- Dna polymerase ii small subunit (74 kDa, Protein from Pyrococcus abyssi GE5)
- Dna (5'-d(*p*cp*cp*gp*gp*gp*cp*cp*gp*ap*gp*cp*cp*gp*tp*(gs)p*(g7p)p*(pst)p*(pst)p*(pst))-3') (6 kDa, DNA from Pyrococcus abyssi GE5)
- Dna polymerase sliding clamp (29 kDa, Protein from Pyrococcus abyssi GE5)
- Binary complex of pold bound to pcna and a primer/template duplex containing three consecutive mismatches (Complex from Pyrococcus abyssi GE5)
- Dp2 (144 kDa, Protein from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- Dna (5'-d(p*ap*gp*cp*ap*cp*gp*gp*cp*tp*cp*gp*gp*cp*cp*cp*gp*g)-3') (7 kDa, DNA from Pyrococcus abyssi GE5)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8thk
Q-score: 0.568
Su M, Wang J, Xiang G, Do HN, Levitz J, Miao Y, Huang XY
Nat Commun (2023) 14 pp. 4819-4819 [ Pubmed: 37563160 DOI: doi:10.1038/s41467-023-40524-2 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- A61603-bound alpha-1a-adrenergic receptor in complex with heterotrimeric gq-protein (160 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- N-[(5s)-5-(4,5-dihydro-1h-imidazol-2-yl)-2-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl]methanesulfonamide (309 Da, Ligand)
- Single fab chain (scfv16) (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-2,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Endolysin,alpha-1a adrenergic receptor (56 kDa, Protein from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8thl
Q-score: 0.464
Su M, Wang J, Xiang G, Do HN, Levitz J, Miao Y, Huang XY
Nat Commun (2023) 14 pp. 4819-4819 [ Pubmed: 37563160 DOI: doi:10.1038/s41467-023-40524-2 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- L-epinephrine (183 Da, Ligand)
- Single fab chain (scfv16) (28 kDa, Protein from Homo sapiens)
- Epinephrine-bound alpha-1a-adrenergic receptor in complex with heterotrimeric gq-protein (160 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-2,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Endolysin,alpha-1a adrenergic receptor (56 kDa, Protein from Homo sapiens)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8dcr
Q-score: 0.593
Su M, Paknejad N, Zhu L, Wang J, Do HN, Miao Y, Liu W, Hite RK, Huang XY
Nat Commun (2022) 13 pp. 4095-4095 [ DOI: doi:10.1038/s41467-022-31823-1 Pubmed: 35835792 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Bos taurus)
- Endolysin,endolysin,beta-1 adrenergic receptor chimera (57 kDa, Protein from Meleagris gallopavo)
- Dobutamine-bound beta1-adrenergic receptor in complex with heterotrimeric gs-protein (Complex from Meleagris gallopavo)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Bos taurus)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Dobutamine (301 Da, Ligand)