cryo-EM structure of Gs-coupled zebrafish GPR4 at pH 6.5
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8zfz
Q-score: 0.41
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Zgpr4-gs complex (Complex from Danio rerio)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
cryo-EM structure of zebrafish GPR4-Gs complex at pH 8.5
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9j31
Q-score: 0.467
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Zgpr4-gs complex (Complex from Danio rerio)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Scfv16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8kh5
Q-score: 0.514
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Cryo-em structure of the gpr174 and mini-gs complex with nb35 (Complex)
- Probable g-protein coupled receptor 174 (39 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- O-{hydroxy[((2r)-2-hydroxy-3-{[(1s)-1-hydroxypentadecyl]oxy}propyl)oxy]phosphoryl}-l-serine (485 Da, Ligand)
- Gs complex (Complex from Homo sapiens)
- Gpr174 (Complex from Homo sapiens)
Cryo-EM structure of the GPR61-Gs complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8kgk
Q-score: 0.469
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Gpr61 and mini-gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr61 and mini-gs complex with nb35 (Complex)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 61 (42 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR161-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8kh4
Q-score: 0.48
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr161 and mini-gs complex with nb35 (Complex from Homo sapiens)
- G-protein coupled receptor 161 (41 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)