cryo-EM structure of Gs-coupled zebrafish GPR4 at pH 6.5
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8zfz
Q-score: 0.41
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Zgpr4-gs complex (Complex from Danio rerio)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
cryo-EM structure of zebrafish GPR4-Gs complex at pH 8.5
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9j31
Q-score: 0.467
Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G
Nat Commun (2025) 16 pp. 605-605 [ DOI: doi:10.1038/s41467-025-55901-2 Pubmed: 39799123 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Zgpr4-gs complex (Complex from Danio rerio)
- Soluble cytochrome b562,g-protein coupled receptor 4,lgbit (72 kDa, Protein from synthetic construct)
- Scfv16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8hj1
Q-score: 0.445
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (39 kDa, Protein from Homo sapiens)
- Gpr21(wt) with gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
FSHR-Follicle stimulating hormone-compound 716340-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8i2g
Q-score: 0.363
Duan J, Xu P, Zhang H, Luan X, Yang J, He X, Mao C, Shen DD, Ji Y, Cheng X, Jiang H, Jiang Y, Zhang S, Zhang Y, Xu HE
Nat Commun (2023) 14 pp. 519-519 [ DOI: doi:10.1038/s41467-023-36170-3 Pubmed: 36720854 ]
- Follicle-stimulating hormone receptor (76 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Follitropin subunit beta (12 kDa, Protein from Homo sapiens)
- Fshr-follicle stimulating hormone-compound 716340-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- N-tert-butyl-n-ethyl-8-methoxy-9-propan-2-yloxy-1-thiophen-2-yl-5,6-dihydroimidazo[5,1-a]isoquinoline-3-carboxamide (467 Da, Ligand)
- Nb35 (13 kDa, Protein from Lama glama)
- Chimera of guanine nucleotide-binding protein g(s) subunit alpha and guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (30 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Myristic acid (228 Da, Ligand)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8hix
Q-score: 0.489
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Gpr21 and gs protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8hj2
Q-score: 0.425
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 21 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gpr21(wt) with g15 (Complex from Homo sapiens)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8hj0
Q-score: 0.466
Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J, Zhao S, Xu F
Nat Commun (2023) 14 pp. 216-216 [ DOI: doi:10.1038/s41467-023-35882-w Pubmed: 36639690 ]
- Nb35 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gpr21 m5 with g15 (Complex from Homo sapiens)
- Probable g-protein coupled receptor 21 (38 kDa, Protein from Homo sapiens)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Water (18 Da, Ligand)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Gpr174/gs complex (Complex from Homo sapiens)
Human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 7vqx
Q-score: 0.543
Xu Y, Feng W, Zhou Q, Liang A, Li J, Dai A, Zhao F, Yan J, Chen CW, Li H, Zhao LH, Xia T, Jiang Y, Xu HE, Yang D, Wang MW
Nat Commun (2022) 13 pp. 2272-2272 [ Pubmed: 35477937 DOI: doi:10.1038/s41467-022-30041-z ]
- Cryo-em structure of the human vasoactive intestinal polypeptide receptor 2 in commplex with pacap27 and g protein (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Human vasoactive intestinal polypeptide receptor 2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex)
- Guanine nucleotide-binding protein g(i) /g(s) /g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody-35 (Complex)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Vasoactive intestinal polypeptide receptor 2 (64 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase activating peptide-27 (Complex from Bos taurus)
- Pituitary adenylate cyclase-activating polypeptide 27 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) /g(s) /g(t) subunit beta-1 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 7wbj
Q-score: 0.425
Xu Y, Feng W, Zhou Q, Liang A, Li J, Dai A, Zhao F, Yan J, Chen CW, Li H, Zhao LH, Xia T, Jiang Y, Xu HE, Yang D, Wang MW
Nat Commun (2022) 13 pp. 2272-2272 [ Pubmed: 35477937 DOI: doi:10.1038/s41467-022-30041-z ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Vasoactive intestinal polypeptide receptor 2 (49 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human vasoactive intestinal polypeptide receptor 2 in commplex with pacap27 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Nanobody-35 (Complex)
- Vasoactive intestinal polypeptide receptor 2 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from synthetic construct)
- Pituitary adenylate cyclase-activating polypeptide 27 (Complex from Bos taurus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short/g(i)/g(s)/g(o) subunit gamma-2 (Complex from Rattus norvegicus)
- Pituitary adenylate cyclase-activating polypeptide 27 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)