Cryo-EM structure of the xGPR4-Gs complex in pH6.2
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8zd1
Q-score: 0.567
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (31 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cryo-em structure of the xgpr4-gs complex in ph6.2 (Complex from Homo sapiens)
Cryo-EM structure of the xGPR4-Gs complex in pH6.7
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8zf6
Q-score: 0.53
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the xgpr4-gs complex in ph6.7 (Complex from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the mmGPR4-Gs complex in pH7.2
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8zf9
Q-score: 0.558
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the mmgpr4-gs complex in ph7.2 (Complex from Mus musculus)
- Nanobody35 (16 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the xtGPR4-Gs complex in pH7.2
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8zfa
Q-score: 0.483
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the xtgpr4-gs complex in ph7.2 (Complex from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the mmGPR4-Gs complex in pH7.6
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8zfc
Q-score: 0.563
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the mmgpr4-gs complex in ph7.6 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the g1:Ox-bound human GLP-1R-Gs complex
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 9j1p
Q-score: 0.505
Fan S, Li J, Zhuang J, Zhou Q, Mai Y, Lin B, Wang MW, Wu C
J Am Chem Soc (2025) 147 pp. 4821-4832 [ Pubmed: 39688263 DOI: doi:10.1021/jacs.4c12808 ]
- Nanobody-35 (15 kDa, Protein from Lama glama)
- Cryo-em structure of the g1 ox-bound human glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- G1:ox (5 kDa, Protein from synthetic construct)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
Cryo-EM structure of the mmGPR4-Gs complex in pH6.2
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 9jvg
Q-score: 0.49
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the mmgpr4-gs complex in ph6.2 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GLP-1(9-36)-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9ivg
Q-score: 0.48
Li J, Li G, Mai Y, Liu X, Yang D, Zhou Q, Wang MW
Acta Pharm Sin B (2024) 14 pp. 5069-5073 [ DOI: doi:10.1016/j.apsb.2024.09.002 Pubmed: 39664415 ]
- Cryo-em structure of the glp-1(9-36)-bound human glp-1r-gs complex (Complex from Homo sapiens)
- Glp-1(9-36) (3 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.07.547762 Pubmed: 37461561 ]
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)