Cryo-EM structure of the xGPR4-Gs complex in pH6.2
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8zd1
Q-score: 0.567
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (31 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cryo-em structure of the xgpr4-gs complex in ph6.2 (Complex from Homo sapiens)
Cryo-EM structure of the xGPR4-Gs complex in pH6.7
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8zf6
Q-score: 0.53
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the xgpr4-gs complex in ph6.7 (Complex from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the mmGPR4-Gs complex in pH7.2
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8zf9
Q-score: 0.558
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the mmgpr4-gs complex in ph7.2 (Complex from Mus musculus)
- Nanobody35 (16 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the xtGPR4-Gs complex in pH7.2
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8zfa
Q-score: 0.483
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the xtgpr4-gs complex in ph7.2 (Complex from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the mmGPR4-Gs complex in pH7.6
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8zfc
Q-score: 0.563
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the mmgpr4-gs complex in ph7.6 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the mmGPR4-Gs complex in pH6.2
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 9jvg
Q-score: 0.49
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- G-protein coupled receptor 4 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the mmgpr4-gs complex in ph6.2 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the ZH8651-bound hTAAR1-Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8wc8
Q-score: 0.533
Shang P, Rong N, Jiang JJ, Cheng J, Zhang MH, Kang D, Qi L, Guo L, Yang GM, Liu Q, Zhou Z, Li XB, Zhu KK, Meng QB, Han X, Yan W, Kong Y, Yang L, Wang X, Lei D, Feng X, Liu X, Yu X, Wang Y, Li Q, Shao ZH, Yang F, Sun JP
Cell (2023) 186 pp. 5347-5362.e24 [ Pubmed: 37963465 DOI: doi:10.1016/j.cell.2023.10.014 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Cryo-em structure of the zh8651-bound htaar1-gs complex (Complex from Homo sapiens)
- Trace amine-associated receptor 1 (39 kDa, Protein from Homo sapiens)
- 2-(4-bromophenyl)ethanamine (200 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f4f
Q-score: 0.509
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Melanocortin-1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein and scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Afamelanotide (Complex)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7f4h
Q-score: 0.52
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Scfv16 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Afamelanotide (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein, nb35 and scfv16 (Complex from Homo sapiens)
Cryo-EM structure of SKF-83959-bound dopamine receptor 1 in complex with Gs protein
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7jvp
Q-score: 0.485
Zhuang Y, Xu P, Mao C, Wang L, Krumm B, Zhou XE, Huang S, Liu H, Cheng X, Huang XP, Shen DD, Xu T, Liu YF, Wang Y, Guo J, Jiang Y, Jiang H, Melcher K, Roth BL, Zhang Y, Zhang C, Xu HE
Cell (2021) 184 pp. 931-942.e18 [ DOI: doi:10.1016/j.cell.2021.01.027 Pubmed: 33571431 ]
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from synthetic construct)
- Cholesterol (386 Da, Ligand)
- D(1a) dopamine receptor (55 kDa, Protein from Homo sapiens)
- Skf-81297-bound dopamine receptor 1 in complex with gi protein (Complex from Homo sapiens)
- (1r)-6-chloro-3-methyl-1-(3-methylphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (317 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)