Cryo-EM structure of a class A orphan GPCR
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7vuh
Q-score: 0.409
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7vui
Q-score: 0.389
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7vuj
Q-score: 0.212
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Chimera endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Magnesium ion (24 Da, Ligand)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
Human Neurokinin 1 receptor (NK1R) substance P Gq chimera (mGsqi) complex
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 7p00
Q-score: 0.546
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Substance p (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gq chimera (gsqi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Human Neurokinin 1 receptor (NK1R) substance P Gs complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7p02
Q-score: 0.502
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Protachykinin-1 (1 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gs chimera (gsi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7piu
Q-score: 0.6
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Calcium ion (40 Da, Ligand)
- Camelid antibody fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Ternary complex of setmelanotide-activated melanocortin 4 receptor with heterotrimeric gs, further stabilized by addition of nanobody 35 (144 MDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Setmelanotide (other names rm-493; bim-22493; irc-022493; imcivree) (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 7piv
Q-score: 0.539
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Ndp-alpha-msh (other names afamelanotide; scenesse) (1 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Ternary complex of the ndp-alpha-msh activated melanocortin 4 receptor (mc4r) gs protein complex stabilized by nanobody 35 (144 MDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Camelid antibody vhh fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi/s chimera protein
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7s0g
Q-score: 0.409
Alegre KO, Paknejad N, Su M, Lou JS, Huang J, Jordan KD, Eng ET, Meyerson JR, Hite RK, Huang XY
Nat Struct Mol Biol (2021) 28 pp. 936-944 [ Pubmed: 34759376 DOI: doi:10.1038/s41594-021-00679-2 ]
- Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric gi/s chimera protein (146 kDa, Complex from Bos taurus)
- Isoprenaline (211 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Beta1-adrenergic receptor (57 kDa, Protein from Meleagris gallopavo)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short chimera (43 kDa, Protein from Bos taurus)
CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 7s8l
Q-score: 0.602
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7s8n
Q-score: 0.579
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)