HCMV Pentamer in complex with CS2pt1p2_A10L Fab and CS3pt1p4_C1L Fab
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8tea
Q-score: 0.377
Zehner M, Alt M, Ashurov A, Goldsmith JA, Spies R, Weiler N, Lerma J, Gieselmann L, Stohr D, Gruell H, Schultz EP, Kreer C, Schlachter L, Janicki H, Laib Sampaio K, Stegmann C, Nemetchek MD, Dahling S, Ullrich L, Dittmer U, Witzke O, Koch M, Ryckman BJ, Lotfi R, McLellan JS, Krawczyk A, Sinzger C, Klein F
Immunity (2023) 56 pp. 2602-2620.e10 [ Pubmed: 37967532 DOI: doi:10.1016/j.immuni.2023.10.009 ]
- Envelope protein ul128 (18 kDa, Protein from Human betaherpesvirus 5)
- Hcmv pentamer in complex with cs2pt1p2_a10l fab and cs3pt1p4_c1l fab (Complex from Human betaherpesvirus 5)
- Ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Cs2pt1p2_a10l fab light chain (22 kDa, Protein from Homo sapiens)
- Cs3pt1p4_c1l fab light chain (23 kDa, Protein from Homo sapiens)
- Cs3pt1p4_c1l fab heavy chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein ul130 (24 kDa, Protein from Human betaherpesvirus 5)
- Cs2pt1p2_a10l fab heavy chain (25 kDa, Protein from Homo sapiens)
Global reconstruction for HCMV Pentamer in complex with CS2pt1p2_A10L Fab and CS3pt1p4_C1L Fab
Zehner M, Alt M, Ashurov A, Goldsmith JA, Spies R, Weiler N, Lerma J, Gieselmann L, Stohr D, Gruell H, Schultz EP, Kreer C, Schlachter L, Janicki H, Laib Sampaio K, Stegmann C, Nemetchek MD, Dahling S, Ullrich L, Dittmer U, Witzke O, Koch M, Ryckman BJ, Lotfi R, McLellan JS, Krawczyk A, Sinzger C, Klein F
Immunity (2023) 56 pp. 2602-2620.e10 [ Pubmed: 37967532 DOI: doi:10.1016/j.immuni.2023.10.009 ]
Zehner M, Goldsmith JG, McLellan JS, Klein F
To Be Published
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7t4r
Q-score: 0.429
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Complex of 2x hcmv pentamer gh, gl, ul128, ul130, ul131a bound to 1x thbd and 2x fabs of human neutralizing antibodies msl-109 and 13h11 (590 kDa, Complex)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Thrombomodulin (55 kDa, Protein from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Hcmv envelope glycoprotein ul131a (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein l (Protein from Human betaherpesvirus 5)
- Neuropilin-2 (Protein from Homo sapiens)
- Fab 8i21 heavy chain (Protein from Homo sapiens)
- Fab 13h11 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul128 (Protein from Human betaherpesvirus 5)
- Fab 8i21 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein h (Protein from Human betaherpesvirus 5)
- Complex of 2x hcmv pentamer proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and 2x fabs of human neutralizing antibodies 8i21 and 13h11 (720 kDa, Complex)
- Fab 13h11 heavy chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul130 (Protein from Human betaherpesvirus 5)
Cryo-EM structure of the HCMV pentamer bound by Fabs 2-18 and 8I21
Resolution: 4.02 Å
EM Method: Single-particle
Fitted PDBs: 7kbb
Q-score: 0.274
Wrapp D, Ye X, Ku Z, Su H, Jones HG, Wang N, Mishra AK, Freed DC, Li F, Tang A, Li L, Jaijyan DK, Zhu H, Wang D, Fu TM, Zhang N, An Z, McLellan JS
Sci Adv (2022) 8 pp. eabm2546-eabm2546 [ Pubmed: 35275718 DOI: doi:10.1126/sciadv.abm2546 ]
- 2-18 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Fab 8i21 (Complex from Homo sapiens)
- Hcmv pentamer bound by antibodies 2-18 and 8i21 (280 kDa, Complex)
- Envelope glycoprotein ul130 (21 kDa, Protein from Human cytomegalovirus)
- Fab 2-18 (Complex from Homo sapiens)
- Envelope protein ul128 (16 kDa, Protein from Human cytomegalovirus)
- Ul131a (13 kDa, Protein from Human cytomegalovirus)
- 8i21 fab light chain (23 kDa, Protein from Homo sapiens)
- 8i21 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Hcmv pentamer (refinement focused on three of the subunits) (Complex from Human betaherpesvirus 5)
- 2-18 fab light chain (23 kDa, Protein from Homo sapiens)
Cryo-EM structure of the HCMV pentamer bound by human neuropilin 2
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 7m22
Q-score: 0.409
Wrapp D, Ye X, Ku Z, Su H, Jones HG, Wang N, Mishra AK, Freed DC, Li F, Tang A, Li L, Jaijyan DK, Zhu H, Wang D, Fu TM, Zhang N, An Z, McLellan JS
Sci Adv (2022) 8 pp. eabm2546-eabm2546 [ Pubmed: 35275718 DOI: doi:10.1126/sciadv.abm2546 ]
- Domains a1a2b1b2 of human neuropilin 2 (Complex from Homo sapiens)
- Envelope glycoprotein ul130 (21 kDa, Protein from Human cytomegalovirus)
- Envelope protein ul128 (16 kDa, Protein from Human cytomegalovirus)
- Ul131a (13 kDa, Protein from Human cytomegalovirus)
- The ectodomain of the hcmv pentamer (Complex from Human betaherpesvirus 5)
- The ectodomain of the hcmv pentamer bound by domains a1a2b1b2 of human neuropilin 2 (230 kDa, Complex)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuropilin-2 (65 kDa, Protein from Homo sapiens)
Cryo-EM structure of the HCMV pentamer bound by antibodies 1-103, 1-32 and 2-25
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7m30
Q-score: 0.323
Wrapp D, Ye X, Ku Z, Su H, Jones HG, Wang N, Mishra AK, Freed DC, Li F, Tang A, Li L, Jaijyan DK, Zhu H, Wang D, Fu TM, Zhang N, An Z, McLellan JS
Sci Adv (2022) 8 pp. eabm2546-eabm2546 [ Pubmed: 35275718 DOI: doi:10.1126/sciadv.abm2546 ]
- Quaternary complex of the hcmv pentamer bound by fabs 1-103, 1-32, and 2-25 (310 kDa, Complex)
- 2-25 fab (Complex from Homo sapiens)
- 1-103 fab heavy chain (24 kDa, Protein from Homo sapiens)
- 1-103 fab light chain (22 kDa, Protein from Homo sapiens)
- 1-103 fab (Complex from Homo sapiens)
- 1-32 fab light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein ul130 (21 kDa, Protein from Human cytomegalovirus)
- 2-25 fab light chain (22 kDa, Protein from Homo sapiens)
- Hcmv pentamer (Complex from Human betaherpesvirus 5)
- 2-25 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (16 kDa, Protein from Human cytomegalovirus)
- Ul131a (13 kDa, Protein from Human cytomegalovirus)
- 1-32 fab heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope glycoprotein l (27 kDa, Protein from Human cytomegalovirus)
- 1-32 fab (Complex from Homo sapiens)
