CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9eqg
Q-score: 0.627
Lyu Q, Xue W, Liu R, Ma Q, Kasaragod VB, Sun S, Li Q, Chen Y, Yuan M, Yang Y, Zhang B, Nie A, Jia S, Shen C, Gao P, Rong W, Yu C, Bi Y, Zhang C, Nan F, Ning G, Rao Z, Yang X, Wang J, Wang W
Nature (2024) 634 pp. 936-943 [ Pubmed: 39261733 DOI: doi:10.1038/s41586-024-07929-5 ]
- Hexadecane (226 Da, Ligand)
- 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
- Hexane (86 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1 (52 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Water (18 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Cryoem structure of human full-length alpha1beta3gamma2l gaba(a)r in complex with gaba and puerarin (250 kDa, Complex from Homo sapiens)
- Chloride ion (35 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Decane (142 Da, Ligand)
- [(2r)-1-octadecanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8z)-icosa-5,8,11,14-tetraenoate (1 kDa, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Puerarin (416 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7pbz
Q-score: 0.548
Kasaragod VB, Mortensen M, Hardwick SW, Wahid AA, Dorovykh V, Chirgadze DY, Smart TG, Miller PS
Nature (2022) 602 pp. 529-533 [ Pubmed: 35140402 DOI: doi:10.1038/s41586-022-04402-z ]
- Hexane (86 Da, Ligand)
- Gaba-a receptor - megabody 25 complex (220 kDa, Complex)
- Gamma-aminobutyric acid receptor subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Megabody 25 (56 kDa, Protein from Lama glama)
- N-octane (114 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (51 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histamine (111 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Decane (142 Da, Ligand)
- Gaba-a receptor (Complex from Homo sapiens)
- Undecane (156 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (47 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Megabody 25 (Complex from Lama glama)
GABA-A receptor bound by a-Cobratoxin
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7pc0
Q-score: 0.489
Kasaragod VB, Mortensen M, Hardwick SW, Wahid AA, Dorovykh V, Chirgadze DY, Smart TG, Miller PS
Nature (2022) 602 pp. 529-533 [ Pubmed: 35140402 DOI: doi:10.1038/s41586-022-04402-z ]
- Gaba-a receptor pentamer bound by cobratoxin (240 kDa, Complex)
- Hexane (86 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Gaba-a receptor pentamer (Complex from Homo sapiens)
- Megabody 25 (56 kDa, Protein from Lama glama)
- Gamma-aminobutyric acid receptor subunit beta-3 (51 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Alpha-cobratoxin (7 kDa, Protein from Naja kaouthia)
- Histamine (111 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (47 kDa, Protein from Homo sapiens)
- Cobratoxin (Complex from Naja kaouthia)
- Megabody 25 (Complex from Lama glama)