Cryo-EM structure of human HGSNAT bound with Acetyl-CoA
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8vkj
Q-score: 0.562
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Complex from Homo sapiens)
- Acetyl coenzyme *a (809 Da, Ligand)
Cryo-EM structure of human HGSNAT bound with Acetyl-CoA and substrate analog
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8vlg
Q-score: 0.523
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Complex from Homo sapiens)
- Acetyl coenzyme *a (809 Da, Ligand)
- 4-methyl-2-oxo-2h-1-benzopyran-7-yl 2-amino-2-deoxy-beta-d-glucopyranoside (337 Da, Ligand)
Cryo-EM structure of human HGSNAT bound with CoA and product analog
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8vli
Q-score: 0.513
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Complex from Homo sapiens)
- 4-methyl-2-oxo-2h-1-benzopyran-7-yl 2-acetamido-2-deoxy-beta-d-glucopyranoside (379 Da, Ligand)
- Coenzyme a (767 Da, Ligand)
Cryo-EM structure of human HGSNAT bound with CoA
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8vlu
Q-score: 0.533
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- Coenzyme a (767 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Complex from Homo sapiens)
Cryo-EM structure of human HGSNAT in inactive state
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8vlv
Q-score: 0.484
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Cellular component from Homo sapiens)
Cryo-EM structure of human HGSNAT in transition state
Resolution: 3.61 Å
EM Method: Single-particle
Fitted PDBs: 8vly
Q-score: 0.494
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun (2024) 15 pp. 5388-5388 [ DOI: doi:10.1038/s41467-024-49614-1 Pubmed: 38918376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heparan-alpha-glucosaminide n-acetyltransferase (73 kDa, Protein from Homo sapiens)
- Purified human hgsnat (73 kDa, Cellular component from Homo sapiens)