Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yi0
Q-score: 0.333
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Transcriptional regulatory protein sin3 (175 kDa, Protein from Saccharomyces cerevisiae S288C)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Rpd3s complex (523 MDa, Complex from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone deacetylase rpd3 (48 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM map of Eaf3 CHD bound to H3K36me3 nucleosome
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7yi1
Q-score: 0.572
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Eaf3 (Complex from Xenopus laevis)
- Dna (Complex from Saccharomyces cerevisiae S288C)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h4/h2a/h2b 1.1 (Complex from synthetic construct)
- Histone h3 (Complex)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Rpd3s complex with h3k36me3 nucleosome (723 MDa, Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
Cryo-EM map of Rpd3S in loose-state Rpd3S-NCP complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yi2
Q-score: 0.483
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Transcriptional regulatory protein sin3 (175 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (Complex from Saccharomyces cerevisiae S288C)
- Sin3/rpd3/eaf3/rco1 (Complex)
- Zinc ion (65 Da, Ligand)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Rpd3s complex with h3k36me3 nucleosome (723 MDa, Complex from synthetic construct)
- Histone deacetylase rpd3 (48 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM map of Rpd3S in close-state Rpd3S-NCP complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7yi3
Q-score: 0.521
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Transcriptional regulatory protein sin3 (175 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Rpd3s complex with h3k36me3 nucleosome (723 MDa, Complex from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone deacetylase rpd3 (48 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM map of Rpd3S complex bound to H3K36me3 nucleosome in close state
Resolution: 3.96 Å
EM Method: Single-particle
Fitted PDBs: 7yi4
Q-score: 0.23
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Transcriptional regulatory protein sin3 (175 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (Complex from Xenopus laevis)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Histone h4/h2a/h2b 1.1 (Complex from synthetic construct)
- Rpd3s complex with h3k36me3 nucleosome (723 MDa, Complex from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Histone h3 (Complex)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Sin3/rpd3/eaf3/rco1 (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone deacetylase rpd3 (48 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM msp of Rpd3S complex bound to H3K36me3 nucleosome in loose state
Resolution: 3.96 Å
EM Method: Single-particle
Fitted PDBs: 7yi5
Q-score: 0.254
Guan H, Wang P, Zhang P, Ruan C, Ou Y, Peng B, Zheng X, Lei J, Li B, Yan C, Li H
Nature (2023) 620 pp. 669-675 [ DOI: doi:10.1038/s41586-023-06349-1 Pubmed: 37468628 ]
- Transcriptional regulatory protein sin3 (175 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (Complex from Xenopus laevis)
- Chromatin modification-related protein eaf3 (45 kDa, Protein from Saccharomyces cerevisiae S288C)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Histone h4/h2a/h2b 1.1 (Complex from synthetic construct)
- Rpd3s complex with h3k36me3 nucleosome (723 MDa, Complex from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Histone h3 (Complex)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Wisdom 601 dna (167-mer) (51 kDa, DNA from synthetic construct)
- Transcriptional regulatory protein rco1 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Sin3/rpd3/eaf3/rco1 (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone deacetylase rpd3 (48 kDa, Protein from Saccharomyces cerevisiae S288C)
catalytic core of human telomerase holoenzyme
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7v99
Q-score: 0.247
Wan F, Ding Y, Zhang Y, Wu Z, Li S, Yang L, Yan X, Lan P, Li G, Wu J, Lei M
Cell Res (2021) 31 pp. 1275-1290 [ DOI: doi:10.1038/s41422-021-00586-7 Pubmed: 34782750 ]
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Telomerase reverse transcriptase (127 kDa, Protein from Homo sapiens)
- Telomerase rna component (145 kDa, RNA from Homo sapiens)
- Histone h2b type 1-k (13 kDa, Protein from Homo sapiens)
- Primer dna (7 kDa, DNA from Homo sapiens)
- Catalytic core (Complex from Homo sapiens)
masked histone region in the catalytic core of human telomerase holoenzyme
Wan F, Ding Y, Zhang Y, Wu Z, Li S, Yang L, Yan X, Lan P, Li G, Wu J, Lei M
Cell Res (2021) 31 pp. 1275-1290 [ DOI: doi:10.1038/s41422-021-00586-7 Pubmed: 34782750 ]
- Histone h2b type 1-k (Protein from Homo sapiens)
- Primer dna (DNA from Homo sapiens)
- Telomerase reverse transcriptase (Protein from Homo sapiens)
- Histone h2a type 1-b/e (Protein from Homo sapiens)
- Telomerase rna component (RNA from Homo sapiens)
- Catalytic core (Complex from Homo sapiens)
The mouse cGAS catalytic domain binding to human assembled nucleosome
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 6x59
Q-score: 0.54
Zhao B, Xu P, Rowlett CM, Jing T, Shinde O, Lei Y, West AP, Liu WR, Li P
Nature (2020) 587 pp. 673-677 [ DOI: doi:10.1038/s41586-020-2749-z Pubmed: 32911481 ]
- Dna (45 kDa, DNA from Homo sapiens)
- Cyclic gmp-amp synthase (43 kDa, Protein from Mus musculus)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (45 kDa, DNA from Homo sapiens)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Cgas-nucleosome complex (Complex from Homo sapiens)
The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells
Resolution: 4.36 Å
EM Method: Single-particle
Fitted PDBs: 6x5a
Q-score: 0.265
Zhao B, Xu P, Rowlett CM, Jing T, Shinde O, Lei Y, West AP, Liu WR, Li P
Nature (2020) 587 pp. 673-677 [ DOI: doi:10.1038/s41586-020-2749-z Pubmed: 32911481 ]
- Cyclic gmp-amp synthase (43 kDa, Protein from Mus musculus)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (natural) (45 kDa, DNA from Homo sapiens)
- Dna (natural) (45 kDa, DNA from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Cgas-nucleosome complex (Complex from Homo sapiens)