Structure of CyclinB1 N-terminus bound to the NCP
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9fh9
Q-score: 0.534
Cirillo L, Young R, Veerapathiran S, Roberti A, Martin M, Abubacar A, Perosa C, Coates C, Muhammad R, Roumeliotis TI, Choudhary JS, Alfieri C, Pines J
EMBO J (2024) 43 pp. 4324-4355 [ DOI: doi:10.1038/s44318-024-00194-2 Pubmed: 39143240 ]
- Cyclin b1 ntd bound to the acidic path of the ncp (Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- G2/mitotic-specific cyclin-b1 (2 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h2a type 3 (14 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Dna (145-mer) (45 kDa, DNA from Homo sapiens)
- Dna (145-mer) (45 kDa, DNA from Homo sapiens)
CryoEM Structure INO80core Hexasome complex composite map state1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8oo7
Q-score: 0.556
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Dna (Complex from Synthetic construct)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Hexasome refinement state1
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8ooa
Q-score: 0.525
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
INO80 core bound to hexasome composite map of state 2
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8oop
Q-score: 0.554
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Dna (Complex from Synthetic construct)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
CryoEM Structure INO80core Hexasome complex ATPase-hexasome refinement state 2
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8oos
Q-score: 0.494
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Histones (Complex from Homo sapiens)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Dna (Complex from Synthetic construct)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Ino80 (Complex from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6zhx
Q-score: 0.625
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ DOI: doi:10.1016/j.celrep.2020.108529 Pubmed: 33357431 ]
- Crosslinked complex of alc1 regulatory linker and the nucleosome (206 kDa, Complex)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Histones (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Water (18 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zhy
Q-score: 0.516
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ DOI: doi:10.1016/j.celrep.2020.108529 Pubmed: 33357431 ]
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- A hexasome (nucleosome lacking one of the two h2a-h2b dimers) with its acidic patch bound by the alc1 linker regulatory region. (170 kDa, Complex)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)