Structural basis for the nucleosome binding and chromatin compaction by the linker histone H5
Structural basis for the nucleosome binding and chromatin compaction by the linker histone H5
Cryo-EM structure of Fft3-nucleosome complex with Fft3 bound to SHL+2 position of the nucleosome
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7xyf
Q-score: 0.272
To Be Published
- Dna (167-mer) (44 kDa, DNA from synthetic construct)
- Histone h3 (11 kDa, Protein from Drosophila melanogaster)
- Dna (167-mer) (45 kDa, DNA from synthetic construct)
- Histone h2b (10 kDa, Protein from Drosophila melanogaster)
- Complex of fft3-nucleosome complex with fft3 bound to shl+2 of the nucleosome (class i fft3-nucleosome complex) (Complex from Schizosaccharomyces pombe 972h-)
- Atp-dependent helicase fft3 (78 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Histone h4 (9 kDa, Protein from Drosophila melanogaster)
- Histone h2a (11 kDa, Protein from Drosophila melanogaster)
Cryo-EM structure of Fft3-nucleosome complex with Fft3 bound to SHL+3 position of the nucleosome
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7xyg
Q-score: 0.219
To Be Published
- Histone h2b (13 kDa, Protein from Drosophila melanogaster)
- Complex of fft3-nucleosome complex with fft3 bound to shl+3 of the nucleosome (class ii fft3-nucleosome complex) (Complex from Schizosaccharomyces pombe 972h-)
- Histone h2a (13 kDa, Protein from Drosophila melanogaster)
- Atp-dependent helicase fft3 (104 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Dna (167-mer) (51 kDa, DNA from synthetic construct)
- Histone h3 (15 kDa, Protein from Drosophila melanogaster)
- Histone h4 (11 kDa, Protein from Drosophila melanogaster)
- Dna (167-mer) (51 kDa, DNA from synthetic construct)
Structure of S. cerevisiae Hop1 CBR bound to a nucleosome
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8cww
Q-score: 0.574
Milano CR, Ur SN, Gu Y, Zhang J, Allison R, Brown G, Neale MJ, Tromer EC, Corbett KD, Hochwagen A
EMBO J (2024) 43 pp. 836-867 [ Pubmed: 38332377 DOI: doi:10.1038/s44318-024-00034-3 ]
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Complex of hop1 cbr domain bound to nucleosome (223 kDa, Complex from Saccharomyces cerevisiae)
- Meiosis-specific protein hop1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Widom 601 dna (146-mer) (45 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Widom 601 dna (146-mer) (44 kDa, DNA from Escherichia coli)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
Structure of a Xenopus Nucleosome with Widom 601 DNA
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8cze
Q-score: 0.598
Milano CR, Ur SN, Gu Y, Zhang J, Allison R, Brown G, Neale MJ, Tromer EC, Corbett KD, Hochwagen A
EMBO J (2024) 43 pp. 836-867 [ Pubmed: 38332377 DOI: doi:10.1038/s44318-024-00034-3 ]
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Widom 601 dna (146-mer) (45 kDa, DNA from Escherichia coli)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Widom 601 dna (146-mer) (44 kDa, DNA from Escherichia coli)
- Nucleosome (200 kDa, Complex from Saccharomyces cerevisiae)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
Structure of human cGAS (K394E) bound to the nucleosome
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6y5d
Q-score: 0.195
Pathare GR, Decout A, Gluck S, Cavadini S, Makasheva K, Hovius R, Kempf G, Weiss J, Kozicka Z, Guey B, Melenec P, Fierz B, Thoma NH, Ablasser A
Nature (2020) 587 pp. 668-672 [ Pubmed: 32911482 DOI: doi:10.1038/s41586-020-2750-6 ]
- Histone h2a type 2-a (14 kDa, Protein from Homo sapiens)
- Pentanedial (100 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 2-a (Complex from Homo sapiens)
- Histone h3.2 (11 kDa, Protein from Homo sapiens)
- Dna (153-mer) (47 kDa, DNA from Homo sapiens)
- Dna (153-mer) (46 kDa, DNA from Homo sapiens)
- Cyclic gmp-amp synthase (42 kDa, Protein from Homo sapiens)
- Protein (Complex from Homo sapiens)
- Histone h2b type 1-k (13 kDa, Protein from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Cryo-em structure of cgas-ncp complex (240 kDa, Complex)
Structure of human cGAS (K394E) bound to the nucleosome (focused refinement of cGAS-NCP subcomplex)
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 6y5e
Q-score: 0.43
Pathare GR, Decout A, Gluck S, Cavadini S, Makasheva K, Hovius R, Kempf G, Weiss J, Kozicka Z, Guey B, Melenec P, Fierz B, Thoma NH, Ablasser A
Nature (2020) 587 pp. 668-672 [ Pubmed: 32911482 DOI: doi:10.1038/s41586-020-2750-6 ]
- Pentanedial (100 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Histone h4 (Complex from Homo sapiens)
- Dna (153-mer) (47 kDa, DNA from Homo sapiens)
- Histone h3.2 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 2-c (11 kDa, Protein from Homo sapiens)
- Histone h2a type 2-c (11 kDa, Protein from Homo sapiens)
- Dna (153-mer) (46 kDa, DNA from Homo sapiens)
- Protein (Complex from Homo sapiens)
- Cyclic gmp-amp synthase (42 kDa, Protein from Homo sapiens)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Cryo em structure of cgas-ncp1 complex (240 kDa, Complex)
- Histone h4 (9 kDa, Protein from Homo sapiens)
cryo-EM structure of human H2A.Z.2.2 nucleosome
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6m4d
Q-score: 0.311
Zhou M, Dai L, Li C, Shi L, Huang Y, Guo Z, Wu F, Zhu P, Zhou Z
EMBO J (2021) 40 pp. e105907-e105907 [ Pubmed: 33073403 DOI: doi:10.15252/embj.2020105907 ]
- Histone h2a.v (12 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- H2a.z.2.2 nucleosome (Complex from Homo sapiens)
- Dna (125-mer) (45 kDa, DNA from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (125-mer) (45 kDa, DNA from Homo sapiens)
cryo-EM structure of human H2A.B nucleosome
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6m4g
Q-score: 0.493
Zhou M, Dai L, Li C, Shi L, Huang Y, Guo Z, Wu F, Zhu P, Zhou Z
EMBO J (2021) 40 pp. e105907-e105907 [ Pubmed: 33073403 DOI: doi:10.15252/embj.2020105907 ]
- Histone h4 (11 kDa, Protein from Homo sapiens)
- H2a.b nucleosome (Complex from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
- Dna (93-mer) (45 kDa, DNA from Homo sapiens)
- Dna (93-mer) (45 kDa, DNA from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a-bbd type 2/3 (12 kDa, Protein from Homo sapiens)