Cryo-EM map of human telomerase-DNA-TPP1-POT1 complex (sharpened map)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7qxb
Q-score: 0.278
Sekne Z, Ghanim GE, van Roon AM, Nguyen THD
Science (2022) 375 pp. 1173-1176 [ Pubmed: 35201900 DOI: doi:10.1126/science.abn6840 ]
- Telomeric dna (Complex from Homo sapiens)
- Histones (Complex from Homo sapiens)
- Histone h2b (18 kDa, Protein from Homo sapiens)
- Protection of telomeres protein 1 (71 kDa, Protein from Homo sapiens)
- Telomerase reverse transcriptase (127 kDa, Protein from Homo sapiens)
- Human telomerase rna (145 kDa, RNA from Homo sapiens)
- Adrenocortical dysplasia homolog (mouse), isoform cra_a (49 kDa, Protein from Homo sapiens)
- Tpp1-pot1 (Complex from Homo sapiens)
- Telomerase reverse transcriptase and telomeric rna (Complex from Homo sapiens)
- Telomeric dna (9 kDa, DNA from Homo sapiens)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Complex of telomeric dna-bound human telomerase with tpp1-pot1 (Complex)
cryo-EM Structure of Nucleosome containing mouse histone variant H2A.Z
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7m1x
Q-score: 0.391
Lewis TS, Sokolova V, Jung H, Ng H, Tan D
Nucleic Acids Res (2021) 49 pp. 11379-11391 [ Pubmed: 34643712 DOI: doi:10.1093/nar/gkab907 ]
- Histone variant h2a.z (Complex from Mus musculus)
- Histone h4 (Complex from Xenopus laevis)
- Histone h2b (Complex from Xenopus laevis)
- Histone h2a.z (13 kDa, Protein from Mus musculus)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (136-mer) (45 kDa, DNA from unidentified)
- Dna (136-mer) (45 kDa, DNA from unidentified)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Nucleosome core particle containing variant h2a.z and canonical core histones (Complex)
- Histone h3 (Complex from Xenopus laevis)
- Dna (136-mer) (Complex from unidentified)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
Nucleosome isolated from metaphase chromosome formed in Xenopus egg extract (oligo fraction)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7kbe
Q-score: 0.445
Arimura Y, Shih RM, Froom R, Funabiki H
Mol Cell (2021) 81 pp. 4377- [ Pubmed: 34478647 DOI: doi:10.1016/j.molcel.2021.08.010 ]
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (157-mer) (47 kDa, DNA from Xenopus laevis)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Nucleosome isolated from metaphase chromosome formed in xenopus egg extract (oligo fraction) (Complex from Xenopus laevis)
- Dna (157-mer) (48 kDa, DNA from Xenopus laevis)
The catalytic core lobe of human telomerase in complex with a telomeric DNA substrate
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7bg9
Q-score: 0.346
Ghanim GE, Fountain AJ, van Roon AM, Rangan R, Das R, Collins K, Nguyen THD
Nature (2021) 593 pp. 449-453 [ Pubmed: 33883742 DOI: doi:10.1038/s41586-021-03415-4 ]
- Telomerase reverse transcriptase,telomerase reverse transcriptase (149 kDa, Protein from Homo sapiens)
- Histone h2b (18 kDa, Protein from Homo sapiens)
- Histone (Complex from Homo sapiens)
- Human telomerase catalytic core in complex with a telomeric dna substrate (Complex)
- Rna (256-mer) (145 kDa, RNA from Homo sapiens)
- Telomerase reverse transcriptase (Complex from Homo sapiens)
- Dna (5'-d(p*tp*tp*ap*gp*gp*g)-3') (5 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Histone h2a (14 kDa, Protein from Homo sapiens)
Structure of human Sox11 transcription factor in complex with a nucleosome
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6t7a
Q-score: 0.37
Dodonova SO, Zhu F, Dienemann C, Taipale J, Cramer P
Nature (2020) 580 pp. 669-672 [ DOI: doi:10.1038/s41586-020-2195-y Pubmed: 32350470 ]
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Structure of human sox11 transcription factor in complex with a nucleosome (218 kDa, Complex)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1-b/e (16 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (13 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Sox11 transcription factor, histones (Complex from Homo sapiens)
- Transcription factor sox-11 (12 kDa, Protein from Homo sapiens)
SAGA-TBP filtered to show the position of the DUB domain
Resolution: 20.0 Å
EM Method: Single-particle
Fitted PDBs: 6tbm
Q-score: 0.08
Papai G, Frechard A, Kolesnikova O, Crucifix C, Schultz P, Ben-Shem A
Nature (2020) 577 pp. 711-716 [ DOI: doi:10.1038/s41586-020-1944-2 Pubmed: 31969704 ]
- Tata-box binding protein (tbp) (Complex from Komagataella phaffii GS115)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- Tata-box binding protein (tbp) (27 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Transcription and mrna export factor sus1 (11 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Transcription initiation factor tfiid subunit 10 (23 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Saga component (Complex from Komagataella phaffii GS115)
- Saga complex (Complex from Komagataella phaffii GS115)
- Transcriptional regulator involved in glucose repression of gal4p-regulated genes (81 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Ubiquitin carboxyl-terminal hydrolase (56 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit of the saga and saga-like transcriptional regulatory complexes, interacts with spt15p to act (39 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit of the saga transcriptional regulatory complex, involved in proper assembly of the complex (136 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Spt8 (34 kDa, Protein from Komagataella phaffii GS115)
- Spt20 (59 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit (60 kda) of tfiid and saga complexes (54 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit (90 kda) of tfiid and saga complexes (80 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Transcriptional coactivator hfi1/ada1 (49 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit (61/68 kda) of tfiid and saga complexes (66 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Polyubiquitin-b (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Saga bound to tbp (1 MDa, Complex)
- Saga-associated factor 11 (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit of saga histone acetyltransferase complex (79 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Transcription-associated protein (438 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Subunit (17 kda) of tfiid and saga complexes, involved in rna polymerase ii transcription initiation (17 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
CENP-A nucleosome bound by two copies of CENP-C(CD) and one copy CENP-N(NT)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6muo
Q-score: 0.369
Allu PK, Dawicki-McKenna JM, Van Eeuwen T, Slavin M, Braitbard M, Xu C, Kalisman N, Murakami K, Black BE
Curr Biol (2019) 29 pp. 2625-2639.e5 [ Pubmed: 31353180 DOI: doi:10.1016/j.cub.2019.06.062 ]
- Cenp-a chromatin complex bound with cenp-c and cenp-n of ccan kinetochore components (276 kDa, Cellular component from Homo sapiens)
- Histone h2b type 2-f (10 kDa, Protein from Homo sapiens)
- Histone h4 (10 kDa, Protein from Homo sapiens)
- Dna/rna (147-mer) (45 kDa, DNA from Homo sapiens)
- Histone h3-like centromeric protein a (11 kDa, Protein from Homo sapiens)
- Centromere protein c (2 kDa, Protein from Homo sapiens)
- Centromere protein n (25 kDa, Protein from Homo sapiens)
- Dna/rna (147-mer) (45 kDa, DNA from Homo sapiens)
- Histone h2a type 1-c (11 kDa, Protein from Homo sapiens)
The complex of ISWI-nucleosome in the ADP.BeF-bound state
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 6k1p
Q-score: 0.42
Yan L, Wu H, Li X, Gao N, Chen Z
Nat Struct Mol Biol (2019) 26 pp. 258-266 [ DOI: doi:10.1038/s41594-019-0199-9 Pubmed: 30872815 ]
- Isw1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- The complex of iswi-nucleosome in the adp-bef-bound state (300 kDa, Complex)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Beryllium trifluoride ion (66 Da, Ligand)
- Dna (167-mer) (51 kDa, DNA from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nuleosome (Complex from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (167-mer) (51 kDa, DNA from Escherichia coli K-12)
- Dna (Complex from Escherichia coli K-12)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Iswi chromatin-remodeling complex atpase isw1 (123 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Poised-state Dot1L bound to the H2B-Ubiquitinated nucleosome
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6nog
Q-score: 0.356
Worden EJ, Hoffmann NA, Hicks CW, Wolberger C
Cell (2019) 176 pp. 1490-1501.e12 [ Pubmed: 30765112 DOI: doi:10.1016/j.cell.2019.02.002 ]
- Histone core (Complex from Xenopus laevis)
- Ubiquitin (Complex from Homo sapiens)
- H2b-ub nucleosome (Complex)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (47 kDa, Protein from Homo sapiens)
- Dot1l (Complex from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- 601 dna strand 1 (44 kDa, DNA from synthetic construct)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- Ubiquitin (9 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Poised state dot1l in complex with the h2b-ub nucleosome (Complex)
- 601 dna strand 2 (45 kDa, DNA from synthetic construct)
Resolution: 7.9 Å
EM Method: Single-particle
Fitted PDBs: 6j4w
Q-score: 0.123
Ehara H, Kujirai T, Fujino Y, Shirouzu M, Kurumizaka H, Sekine SI
Science (2019) 363 pp. 744-747 [ Pubmed: 30733384 DOI: doi:10.1126/science.aav8912 ]
- Dna (198-mer) (61 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*gp*up*gp*up*up*up*gp*gp*gp*up*gp*gp*up*gp*gp*c)-3') (5 kDa, RNA from synthetic construct)
- Transcription elongation factor spt4 (12 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Magnesium ion (24 Da, Ligand)
- Transcription elongation factor 1 homolog (12 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii elongation complex bound with elf1 and spt4/5, stalled at shl(-5) of the nucleosome (Complex from Komagataella pastoris)
- Rna polymerase subunit abc10-alpha (7 kDa, Protein from Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1))
- Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Zinc ion (65 Da, Ligand)
- Rna polymerase subunit abc10-beta (8 kDa, Protein from Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1))
- Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Dna (198-mer) (60 kDa, DNA from synthetic construct)
- Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Histone h3.3 (15 kDa, Protein from Homo sapiens)
- Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Spt5 (101 kDa, Protein from Komagataella pastoris)
- Rna polymerase subunit abc27, common to rna polymerases i, ii, and iii (24 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))