Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6xh7
Q-score: 0.344
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ Pubmed: 34113812 DOI: doi:10.1016/j.isci.2021.102449 ]
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Cuer-tac without rna (530 kDa, Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
CueR-transcription activation complex with RNA transcript
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6xh8
Q-score: 0.318
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ DOI: doi:10.1016/j.isci.2021.102449 Pubmed: 34113812 ]
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Cuer-tac with rna (540 kDa, Complex from Escherichia coli)
The cryo-EM structure of E. coli CueR transcription activation complex
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 6ldi
Q-score: 0.471
Fang C, Philips SJ, Wu X, Chen K, Shi J, Shen L, Xu J, Feng Y, O'Halloran TV, Zhang Y
Nat Chem Biol (2021) 17 pp. 57-64 [ DOI: doi:10.1038/s41589-020-00653-x Pubmed: 32989300 ]
- Rna (5'-r(*cp*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli cuer transcription activation complex (507 kDa, Complex from Escherichia coli K-12)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Silver ion (107 Da, Ligand)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Hth-type transcriptional regulator cuer (15 kDa, Protein from Escherichia coli (strain K12))
Resolution: 4.22 Å
EM Method: Single-particle
Fitted PDBs: 7c17
Q-score: 0.278
Fang C, Philips SJ, Wu X, Chen K, Shi J, Shen L, Xu J, Feng Y, O'Halloran TV, Zhang Y
Nat Chem Biol (2021) 17 pp. 57-64 [ DOI: doi:10.1038/s41589-020-00653-x Pubmed: 32989300 ]
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli (strain K12))
- Dna (72-mer) (22 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Silver ion (107 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Hth-type transcriptional regulator cuer (15 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli cuer transcription activation complex with fully duplex promoter dna (507 kDa, Complex from Escherichia coli K-12)
- Dna (72-mer) (22 kDa, DNA from synthetic construct)