Cryo-EM structure of Nap1 core
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9b23
Q-score: 0.311
Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM
J Cell Biol (2025) 224 [ DOI: doi:10.1083/jcb.202408193 Pubmed: 39601790 ]
- Nap1 isoform 1 (36 kDa, Protein from Saccharomyces cerevisiae)
- Complex of kap114 bound to nap1 and histone h2a-h2b (72 kDa, Complex from Saccharomyces cerevisiae)
Cryo-EM structure of yeast (Nap1)2-Kap114-H2A-H2B
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9b31
Q-score: 0.324
Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM
J Cell Biol (2025) 224 [ DOI: doi:10.1083/jcb.202408193 Pubmed: 39601790 ]
- Kap114 isoform 1 (114 kDa, Protein from Saccharomyces cerevisiae)
- Crosslinked mixture of kap114 with nap1 and h2a-h2b (72 kDa, Complex from Saccharomyces cerevisiae)
- Histone h2b (14 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a (13 kDa, Protein from Saccharomyces cerevisiae)
- Nap1 isoform 1 (36 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 9b3f
Q-score: 0.21
Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM
J Cell Biol (2025) 224 [ DOI: doi:10.1083/jcb.202408193 Pubmed: 39601790 ]
- Histone h2a (13 kDa, Protein from Saccharomyces cerevisiae)
- Nap1 isoform 1 (36 kDa, Protein from Saccharomyces cerevisiae)
- Kap114 isoform 1 (114 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b (14 kDa, Protein from Saccharomyces cerevisiae)
- Crosslinked mixture of kap114 with nap1 and h2a-h2b (72 kDa, Complex from Saccharomyces cerevisiae)
Locally refined map for Nap1 and H2A-H2B in (Nap1)2-H2A-H2B-Kap114 structure
Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM
J Cell Biol (2025) 224 [ DOI: doi:10.1083/jcb.202408193 Pubmed: 39601790 ]
Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9b3i
Q-score: 0.293
Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM
J Cell Biol (2025) 224 [ DOI: doi:10.1083/jcb.202408193 Pubmed: 39601790 ]
- Gtp-binding nuclear protein (21 kDa, Protein from Saccharomyces cerevisiae)
- Complex of kap114 bound to ran gtpase gsp1, h2a-h2b and nap1 (Complex from Saccharomyces cerevisiae)
- Histone h2b (14 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Histone h2a (13 kDa, Protein from Saccharomyces cerevisiae)
- Kap114 isoform 1 (114 kDa, Protein from Saccharomyces cerevisiae)
- Nap1 isoform 1 (48 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of Kap114 bound to H2A-H2B
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8f0x
Q-score: 0.51
Jiou J, Shaffer JM, Bernades NE, Fung HYJ, Kikumoto Dias J, D'Arcy S, Chook YM
PNAS (2023) 120 pp. e2301199120-e2301199120 [ DOI: doi:10.1073/pnas.2301199120 Pubmed: 37450495 ]
- Histone h2a.2 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Complex of kap114 bound to h2a-h2b dimer (Complex from Saccharomyces cerevisiae S288C)
- Importin subunit beta-5 (114 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8f1e
Q-score: 0.408
Jiou J, Shaffer JM, Bernades NE, Fung HYJ, Kikumoto Dias J, D'Arcy S, Chook YM
PNAS (2023) 120 pp. e2301199120-e2301199120 [ DOI: doi:10.1073/pnas.2301199120 Pubmed: 37450495 ]
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Complex of kap114 bound to gsp1 (rangtp) and h2a-h2b (Complex from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Histone h2a.2 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Gtp-binding nuclear protein gsp1/cnr1 (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Importin subunit beta-5 (114 kDa, Protein from Saccharomyces cerevisiae S288C)