NSD2 bearing E1099K/T1150A dual mutation in complex with 187-bp NCP
Resolution: 3.75 Å
EM Method: Single-particle
Fitted PDBs: 7cro
Q-score: 0.448
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Nsd2 bearing e1099k/t1150a dual mutation in complex with 187-bp ncp (300 kDa, Complex)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Zinc ion (65 Da, Ligand)
- Nsd2 (Complex from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3, h4, h2a and h2b (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase nsd2 (79 kDa, Protein from Homo sapiens)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Dna (Complex from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
NSD3 bearing E1181K/T1232A dual mutation in complex with 187-bp NCP (1:1 binding mode)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7crp
Q-score: 0.542
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Zinc ion (65 Da, Ligand)
- Nsd3 (Complex from Homo sapiens)
- Histone h3, h4, h2a, h2b (Complex from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase nsd3 (85 kDa, Protein from Homo sapiens)
- Nsd3 bearing e1181k/t1232a dual mutation in complex with 187-bp ncp (1:1 binding mode) (300 kDa, Complex)
- Dna (Complex from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
NSD3 bearing E1181K/T1232A dual mutation in complex with 187-bp NCP (2:1 binding mode)
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7crq
Q-score: 0.507
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ DOI: doi:10.1038/s41586-020-03069-8 Pubmed: 33361816 ]
- Nsd3 (Complex from Homo sapiens)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone-lysine n-methyltransferase nsd3 (85 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (Complex from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Nsd3 bearing e1181k/t1232a dual mutation in complex with 187-bp ncp (2:1 binding mode) (300 kDa, Complex)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone h3, h4, h2a, h2b (Complex from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
Native NSD3 bound to 187-bp nucleosome
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7crr
Q-score: 0.464
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Dna (Complex from synthetic construct)
- Dna(168-mer) (57 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Histone-lysine n-methyltransferase nsd3 (85 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histone h3, h4, h2a, h2b (Complex from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Native nsd3 (Complex from Homo sapiens)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Dna (168-mer) (57 kDa, DNA from synthetic construct)
- Cryo-em structure of the native nsd3 bound to 187-bp nucleosome (300 kDa, Complex)
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6ftx
Q-score: 0.23
Sundaramoorthy R, Hughes AL, El-Mkami H, Norman DG, Ferreira H, Owen-Hughes T
eLife (2018) 7 [ DOI: doi:10.7554/eLife.35720 Pubmed: 30079888 ]
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Dna (160-mer) (49 kDa, DNA from synthetic construct)
- Chromatin-remodeling atpase (102 kDa, Protein from Saccharomyces cerevisiae)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Homo sapiens)
- Beryllium trifluoride ion (66 Da, Ligand)
- Histone h3 (11 kDa, Protein from Petromyzon marinus)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- Histone h3.3c (12 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (159-mer) (48 kDa, DNA from synthetic construct)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (450 kDa, Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from synthetic construct)