Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6nzo
Q-score: 0.364
Nat Commun (2019) 10 pp. 3795-3795 [ DOI: doi:10.1038/s41467-019-11726-4 Pubmed: 31439846 ]
- Dna (149-mer) (46 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Dna (149-mer) (45 kDa, DNA from synthetic construct)
- Histone h3, histone h4, ubiquitin-60s ribosomal protein l40,histone h2a, histone h2b 1.1 (Complex from Xenopus laevis)
- Ubiquitin-60s ribosomal protein l40,histone h2a (24 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Set2 nucleosome complex (240 kDa, Complex)
- Histone-lysine n-methyltransferase (Complex from Chaetomium thermophilum)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6px3
Q-score: 0.261
Nat Commun (2019) 10 pp. 3795-3795 [ DOI: doi:10.1038/s41467-019-11726-4 Pubmed: 31439846 ]
- Zinc ion (65 Da, Ligand)
- Set2 bound to ubiquitinated nucleosome (260 kDa, Complex)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Histone h3, histone h4, ubiquitin-60s ribosomal protein l40,histone h2a, histone h2b 1.1 (Complex from Xenopus laevis)
- Dna (145-mer) (45 kDa, DNA from synthetic construct)
- Ubiquitin-60s ribosomal protein l40,histone h2a (24 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (145-mer) (46 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Histone-lysine n-methyltransferase (Complex from Chaetomium thermophilum)
CryoEM Structure INO80core Nucleosome complex
Resolution: 4.34 Å
EM Method: Single-particle
Fitted PDBs: 6fml
Q-score: 0.33
Eustermann S, Schall K, Kostrewa D, Lakomek K, Strauss M, Moldt M, Hopfner KP
Nature (2018) 556 pp. 386-390 [ DOI: doi:10.1038/s41586-018-0029-y Pubmed: 29643509 ]
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ino80 (210 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Synthetic deoxyribonucleic acid (Complex from synthetic construct)
- Ruvb-like helicase (53 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Actin related protein 5 (85 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nucleosomal dna strand 2 (60 kDa, DNA from synthetic construct)
- Histone octamer (Complex from Homo sapiens)
- Ino80core nucleosome complex (1 MDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ino80core (Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Les2 (52 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Ies6 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nucleosomal dna strand 1 (60 kDa, DNA from synthetic construct)
- Ruvb-like helicase (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))