Cryo-EM structure of SNF2h-nucleosome complex (consensus structure)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Snf2h (Complex from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna reverse (DNA from synthetic construct)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna forward (DNA from synthetic construct)
- Nucleosome with xenopus laevis histones (Complex from Xenopus laevis)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (100 kDa, Complex from Homo sapiens)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Histone h3.2 (Protein from Xenopus laevis)
Cryo-EM structure of SNF2h-nucleosome complex (single-bound structure)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna reverse strand (DNA from synthetic construct)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna forward strand (DNA from synthetic construct)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Histone h3.2 (Protein from Xenopus laevis)
Cryo-EM structure of doubly-bound SNF2h-nucleosome complex (conformation 1)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (DNA from synthetic construct)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (DNA from synthetic construct)
- Histone h3.2 (Protein from Xenopus laevis)
Cryo-EM structure of doubly-bound SNF2h-nucleosome complex (conformation 2)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (DNA from synthetic construct)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (DNA from synthetic construct)
- Histone h3.2 (Protein from Xenopus laevis)
Class1 of the INO80-Hexasome complex
Resolution: 6.68 Å
EM Method: Single-particle
Fitted PDBs: 8ett
Q-score: 0.186
Wu H, Munoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y
Science (2023) 381 pp. 319-324 [ DOI: doi:10.1126/science.adf4197 Pubmed: 37384669 ]
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Class1 hexasome in ino80-hexasome complex (Complex from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (110-mer) (70 kDa, DNA from synthetic construct)
- Dna (110-mer) (69 kDa, DNA from synthetic construct)
Class2 of the INO80-Hexasome complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8etv
Q-score: 0.387
Wu H, Munoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y
Science (2023) 381 pp. 319-324 [ DOI: doi:10.1126/science.adf4197 Pubmed: 37384669 ]
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Class2 hexasome in ino80-hexasome complex (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (110-mer) (70 kDa, DNA from synthetic construct)
- Dna (110-mer) (69 kDa, DNA from synthetic construct)
Class3 of the INO80-Hexasome complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8eu2
Q-score: 0.434
Wu H, Munoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y
Science (2023) 381 pp. 319-324 [ DOI: doi:10.1126/science.adf4197 Pubmed: 37384669 ]
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Ino80-hex (Complex from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (110-mer) (70 kDa, DNA from synthetic construct)
- Dna (110-mer) (69 kDa, DNA from synthetic construct)
Class1 of the INO80-Nucleosome complex
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8eue
Q-score: 0.356
Wu H, Munoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y
Science (2023) 381 pp. 319-324 [ DOI: doi:10.1126/science.adf4197 Pubmed: 37384669 ]
- Dna (147-mer) (69 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus)
- Class1 nucleosome of the ino80-nucleosome complex (Complex from Xenopus)
- Dna (147-mer) (70 kDa, DNA from synthetic construct)
- Histone h3.2 (15 kDa, Protein from Xenopus)
- Histone h4 (11 kDa, Protein from Xenopus)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus)
Class2 of the INO80-Nucleosome complex
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8euj
Q-score: 0.322
Wu H, Munoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y
Science (2023) 381 pp. 319-324 [ DOI: doi:10.1126/science.adf4197 Pubmed: 37384669 ]
- Histone h3.2 (15 kDa, Protein from Xenopus)
- Class2 nucleosome of the ino80-nucleosome complex (Complex from Xenopus)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus)
- Dna (147-mer) (70 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus)
- Dna (147-mer) (69 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus)