Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 8t9h
Q-score: 0.393
Abini-Agbomson S, Gretarsson K, Shih RM, Hsieh L, Lou T, De Ioannes P, Vasilyev N, Lee R, Wang M, Simon MD, Armache JP, Nudler E, Narlikar G, Liu S, Lu C, Armache KJ
Mol Cell (2023) 83 pp. 2872-2883.e7 [ DOI: doi:10.1016/j.molcel.2023.07.020 Pubmed: 37595555 ]
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (44 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Suv420h1-h2a.z nucleosome complex (Complex from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Histone-lysine n-methyltransferase kmt5b (44 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8t9f
Q-score: 0.516
Abini-Agbomson S, Gretarsson K, Shih RM, Hsieh L, Lou T, De Ioannes P, Vasilyev N, Lee R, Wang M, Simon MD, Armache JP, Nudler E, Narlikar G, Liu S, Lu C, Armache KJ
Mol Cell (2023) 83 pp. 2872-2883.e7 [ DOI: doi:10.1016/j.molcel.2023.07.020 Pubmed: 37595555 ]
- Dna (122-mer) (44 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Histone h2a.z (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Suv420h1-h2a.z nucleosome complex (Complex from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase kmt5b (44 kDa, Protein from Homo sapiens)
- Dna (122-mer) (45 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8thu
Q-score: 0.508
Abini-Agbomson S, Gretarsson K, Shih RM, Hsieh L, Lou T, De Ioannes P, Vasilyev N, Lee R, Wang M, Simon MD, Armache JP, Nudler E, Narlikar G, Liu S, Lu C, Armache KJ
Mol Cell (2023) 83 pp. 2872-2883.e7 [ DOI: doi:10.1016/j.molcel.2023.07.020 Pubmed: 37595555 ]
- Histone h2a.z (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Suv420h1-h2a.z nucleosome complex (Complex from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
Active state Dot1 bound to the unacetylated H4 nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7k6p
Q-score: 0.473
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Polyubiquitin-b (Complex from Homo sapiens)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
Active state Dot1 bound to the H4K16ac nucleosome
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7k6q
Q-score: 0.489
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Polyubiquitin-b (Complex from Homo sapiens)
- Active state dot1 bound to the h4k16ac nucleosome (Complex from Saccharomyces cerevisiae)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
- Histones (Complex from Xenopus laevis)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (Complex from Synthetic construct)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)