Cryo-EM structure of SSX1 bound to the H2AK119Ub nucleosome at a resolution of 3.05 angstrom
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 8hqy
Q-score: 0.47
Tong Z, Ai H, Xu Z, He K, Chu GC, Shi Q, Deng Z, Xue Q, Sun M, Du Y, Liang L, Li JB, Pan M, Liu L
Nat Struct Mol Biol (2024) 31 pp. 300-310 [ DOI: doi:10.1038/s41594-023-01141-1 Pubmed: 38177667 ]
- Histone h2a type 1-b/e (11 kDa, Protein from Homo sapiens)
- Ssx1-h2ak119ub nucleosome complex (290 kDa, Complex from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Protein ssx2 (2 kDa, Protein from Homo sapiens)
- Dna (137-mer) (41 kDa, DNA from Homo sapiens)
- Polyubiquitin-b (fragment) (8 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (11 kDa, Protein from Homo sapiens)
- Dna (136-mer) (42 kDa, DNA from Homo sapiens)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Histone h3 (11 kDa, Protein from Homo sapiens)
- Histone h4 (fragment) (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of SSX1 bound to the unmodified nucleosome at a resolution of 3.02 angstrom
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8hr1
Q-score: 0.532
Tong Z, Ai H, Xu Z, He K, Chu GC, Shi Q, Deng Z, Xue Q, Sun M, Du Y, Liang L, Li JB, Pan M, Liu L
Nat Struct Mol Biol (2024) 31 pp. 300-310 [ DOI: doi:10.1038/s41594-023-01141-1 Pubmed: 38177667 ]
- Histone h2a type 1-b/e (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Ssx1 (1 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Homo sapiens)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Histone h3 (11 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Homo sapiens)
- Ssx1-unmodified nucleosome complex (280 kDa, Complex from Homo sapiens)
cryo-EM structure of gammaH2AXK15ub-H4K20me2 nucleosome bound to 53BP1
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7yqk
Q-score: 0.424
Ai H, Chu GC, Gong Q, Tong ZB, Deng Z, Liu X, Yang F, Xu Z, Li JB, Tian C, Liu L
J Am Chem Soc (2022) 144 pp. 18329-18337 [ Pubmed: 36166692 DOI: doi:10.1021/jacs.2c06156 ]
- Histone h2b (10 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Udr motif of 53bp1 (2 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Complex of 53bp1(1484-1972) and gammah2axk15ub-h4k20me2 nucleosome (Complex from Homo sapiens)
- Histone h3.1 (11 kDa, Protein from Homo sapiens)
- Histone h2ax (12 kDa, Protein from synthetic construct)
- Tp53-binding protein 1 (13 kDa, Protein from Homo sapiens)
cryo-EM structure of unmodified nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xd1
Q-score: 0.558
Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB, Lou Z, Liu L
Nat Chem Biol (2022) 18 pp. 972-980 [ Pubmed: 35739357 DOI: doi:10.1038/s41589-022-01067-7 ]
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1-b/e (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Histone h3 (11 kDa, Protein from Homo sapiens)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Unmodified nucleosome (Complex from Homo sapiens)