Active state Dot1 bound to the unacetylated H4 nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7k6p
Q-score: 0.473
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histones (Complex from Xenopus laevis)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Polyubiquitin-b (Complex from Homo sapiens)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Dna (146-mer) (Complex from synthetic construct)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
Active state Dot1 bound to the H4K16ac nucleosome
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7k6q
Q-score: 0.489
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Active state dot1 bound to the h4k16ac nucleosome (Complex from Saccharomyces cerevisiae)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Dna (146-mer) (Complex from Synthetic construct)
- Polyubiquitin-b (Complex from Homo sapiens)
- Histones (Complex from Xenopus laevis)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
Noncatalytic conformation Dot1 bound to the unacetylated H4 nucleosome
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (Protein from Xenopus lenduensis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Polyubiquitin-b (Protein from Homo sapiens)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from Synthetic)
- Noncatalytic conformation dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
Active state Dot1 bound to the H4K16ac nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Active state dot1 bound to the h4k16 acetylated nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Dna (146-mer) (Complex from Synthetic)
Active state Dot1 bound to the unacetylated H4 nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Histone h3.2 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Dna (146-mer) (Complex from Synthetic)