Cryo-EM structure of DNMT3A1 UDR in complex with H2AK119Ub-nucleosome
Gretarsson KH, Abini-Agbomson S, Gloor SL, Weinberg DN, McCuiston JL, Kumary VUS, Hickman AR, Sahu V, Lee R, Xu X, Lipieta N, Flashner S, Adeleke OA, Popova IK, Taylor HF, Noll K, Windham CL, Maryanski DN, Venters BJ, Nakagawa H, Keogh MC, Armache KJ, Lu C
Sci Adv (2024) 10 pp. eadp0975-eadp0975 [ Pubmed: 39196936 DOI: doi:10.1126/sciadv.adp0975 ]
- Dna (cytosine-5)-methyltransferase 3a (9 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (44 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Dnmt3a1 udr in complex with h2ak119ub-nucleosome (Complex from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Structure of apo telomeric nucleosome
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8ox0
Q-score: 0.607
Hu H, van Roon AM, Ghanim GE, Ahsan B, Oluwole AO, Peak-Chew SY, Robinson CV, Nguyen THD
Sci Adv (2023) 9 pp. eadi4148-eadi4148 [ Pubmed: 37624885 DOI: doi:10.1126/sciadv.adi4148 ]
- Histone h2b type 1-c/e/f/g/i (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Human telomeric nucleosome (Complex from Homo sapiens)
- Histone h2a type 1-c (14 kDa, Protein from Homo sapiens)
- Trf1core in complex with telomeric nucleosome (Complex)
- Telomeric dna g strand (46 kDa, DNA from Homo sapiens)
- Telomeric dna c strand (43 kDa, DNA from Homo sapiens)
Structure of TRF1core in complex with telomeric nucleosome
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8ox1
Q-score: 0.579
Hu H, van Roon AM, Ghanim GE, Ahsan B, Oluwole AO, Peak-Chew SY, Robinson CV, Nguyen THD
Sci Adv (2023) 9 pp. eadi4148-eadi4148 [ DOI: doi:10.1126/sciadv.adi4148 Pubmed: 37624885 ]
- Telomeric repeat-binding factor 1 (50 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1-c (14 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Human telomeric nucleosome (Complex from Homo sapiens)
- Human trf1core complex (Complex from Homo sapiens)
- Telomeric dna g strand (46 kDa, DNA from Homo sapiens)
- Telomeric repeat-binding factor 1 (50 kDa, Protein from Homo sapiens)
- Telomeric dna c strand (43 kDa, DNA from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (14 kDa, Protein from Homo sapiens)
- Trf1core in complex with telomeric nucleosome (Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7z0o
Q-score: 0.507
Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Muller CW
Sci Adv (2022) 8 pp. eabn5725-eabn5725 [ DOI: doi:10.1126/sciadv.abn5725 Pubmed: 35442737 ]
- Upstream activation factor subunit uaf30 (26 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (45 kDa, DNA from Saccharomyces cerevisiae)
- Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae)
- Non-template dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Rna polymerase i-specific transcription initiation factor rrn5 (41 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae)
- Upstream activating factor (uaf) bound to tbp and promoter dna (Complex from Saccharomyces cerevisiae)
- Rna polymerase i-specific transcription initiation factor rrn10 (16 kDa, Protein from Saccharomyces cerevisiae)
- Rna polymerase i-specific transcription initiation factor rrn9 (42 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)