Cryo-EM structure of yeast SWR1C subunit Swc5 bound to the nucleosome, 3D class 0
Baier AS, Gioacchini N, Eek P, Leith EM, Tan S, Peterson CL
eLife (2024) 13 [ Pubmed: 38809771 DOI: doi:10.7554/eLife.94869 ]
- Swc5 in complex with nucleosome core particle (Complex from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 reverse dna (DNA from synthetic construct)
- Histone h2b.1 (Protein from Saccharomyces cerevisiae)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a.1 (Protein from Saccharomyces cerevisiae)
- Widom 601 forward dna (DNA from synthetic construct)
- Swr1-complex protein 5 (Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast SWR1C subunit Swc5 bound to the nucleosome, 3D class 1
Baier AS, Gioacchini N, Eek P, Leith EM, Tan S, Peterson CL
eLife (2024) 13 [ Pubmed: 38809771 DOI: doi:10.7554/eLife.94869 ]
- Swc5 in complex with nucleosome core particle (Complex from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 reverse dna (DNA from synthetic construct)
- Histone h2b.1 (Protein from Saccharomyces cerevisiae)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a.1 (Protein from Saccharomyces cerevisiae)
- Widom 601 forward dna (DNA from synthetic construct)
- Swr1-complex protein 5 (Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast SWR1C subunit Swc5 bound to the nucleosome, 3D class 2
Baier AS, Gioacchini N, Eek P, Leith EM, Tan S, Peterson CL
eLife (2024) 13 [ Pubmed: 38809771 DOI: doi:10.7554/eLife.94869 ]
- Swc5 in complex with nucleosome core particle (Complex from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 reverse dna (DNA from synthetic construct)
- Histone h2b.1 (Protein from Saccharomyces cerevisiae)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a.1 (Protein from Saccharomyces cerevisiae)
- Widom 601 forward dna (DNA from synthetic construct)
- Swr1-complex protein 5 (Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast SWR1C subunit Swc5 bound to the nucleosome, 3D class 4
Baier AS, Gioacchini N, Eek P, Leith EM, Tan S, Peterson CL
eLife (2024) 13 [ Pubmed: 38809771 DOI: doi:10.7554/eLife.94869 ]
- Swc5 in complex with nucleosome core particle (Complex from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 reverse dna (DNA from synthetic construct)
- Histone h2b.1 (Protein from Saccharomyces cerevisiae)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a.1 (Protein from Saccharomyces cerevisiae)
- Widom 601 forward dna (DNA from synthetic construct)
- Swr1-complex protein 5 (Protein from Saccharomyces cerevisiae)
Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ssa
Q-score: 0.449
Donovan BT, Chen H, Eek P, Meng Z, Jipa C, Tan S, Bai L, Poirier MG
Mol Cell (2023) 83 pp. 1251-1263.e6 [ Pubmed: 36996811 DOI: doi:10.1016/j.molcel.2023.03.006 ]
- Pioneer factor cbf1 in complex with the nucleosome (286 kDa, Complex)
- Dna (Complex from synthetic construct)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (137-mer) (46 kDa, DNA from synthetic construct)
- Histone h2a.1, histone h2b.1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2a.1 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3.2, histone h4 (Complex from Xenopus laevis)
- Dna (137-mer) (45 kDa, DNA from synthetic construct)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Centromere-binding protein 1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6zhx
Q-score: 0.625
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Dna (Complex from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Water (18 Da, Ligand)
- Crosslinked complex of alc1 regulatory linker and the nucleosome (206 kDa, Complex)
- Histones (Complex from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zhy
Q-score: 0.516
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ DOI: doi:10.1016/j.celrep.2020.108529 Pubmed: 33357431 ]
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- A hexasome (nucleosome lacking one of the two h2a-h2b dimers) with its acidic patch bound by the alc1 linker regulatory region. (170 kDa, Complex)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)