Cryo-EM structure of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8b0a
Q-score: 0.483
Bacic L, Gaullier G, Mohapatra J, Mao G, Brackmann K, Panfilov M, Liszczak G, Sabantsev A, Deindl S
Nat Commun (2024) 15 pp. 1000-1000 [ DOI: doi:10.1038/s41467-024-45237-8 Pubmed: 38307862 ]
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
- Complex between alc1 and a site-specifically parylated nucleosome (304 kDa, Complex from Homo sapiens)
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (98 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
Cryo-EM structure of ALC1/CHD1L bound to a PARylated nucleosome
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7otq
Q-score: 0.282
Bacic L, Gaullier G, Sabantsev A, Lehmann LC, Brackmann K, Dimakou D, Halic M, Hewitt G, Boulton S, Deindl S, Workman JL
eLife (2021) 10 [ Pubmed: 34486521 DOI: doi:10.7554/eLife.71420 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Alc1/chd1l bound to a parylated nucleosome (305 kDa, Complex from Xenopus laevis)
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
- Chromodomain-helicase-dna-binding protein 1-like (98 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
cryoDRGN graph traversal of the ALC1 - PARylated nucleosome complex particle distribution
Bacic L, Gaullier G, Sabantsev A, Lehmann LC, Brackmann K, Dimakou D, Halic M, Hewitt G, Boulton S, Deindl S
eLife (2021) 10 [ Pubmed: 34486521 DOI: doi:10.7554/eLife.71420 ]
- Widom 601 sequence (Complex from synthetic construct)
- Histone (Complex from Xenopus laevis)
- Histone h2b (Protein from Xenopus laevis)
- Widom 601 sequence (DNA from synthetic construct)
- Histone h2a (Protein from Xenopus laevis)
- Histone h4 (Protein from Xenopus laevis)
- Histone h3 (Protein from Xenopus laevis)
- Alc1/chd1l bound to a parylated nucleosome (305 kDa, Complex)
- Alc1/chd1l (Protein from Homo sapiens)
- Alc1/chd1l (Complex from Homo sapiens)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6zhx
Q-score: 0.625
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Dna (Complex from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Water (18 Da, Ligand)
- Crosslinked complex of alc1 regulatory linker and the nucleosome (206 kDa, Complex)
- Histones (Complex from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zhy
Q-score: 0.516
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ DOI: doi:10.1016/j.celrep.2020.108529 Pubmed: 33357431 ]
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- A hexasome (nucleosome lacking one of the two h2a-h2b dimers) with its acidic patch bound by the alc1 linker regulatory region. (170 kDa, Complex)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)