Noncatalytic conformation Dot1 bound to the unacetylated H4 nucleosome
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (Protein from Xenopus lenduensis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Polyubiquitin-b (Protein from Homo sapiens)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from Synthetic)
- Noncatalytic conformation dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
Active state Dot1 bound to the H4K16ac nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Active state dot1 bound to the h4k16 acetylated nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Dna (146-mer) (Complex from Synthetic)
Active state Dot1 bound to the unacetylated H4 nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ Pubmed: 33479126 DOI: doi:10.1126/science.abc6663 ]
- Histone h3.2 (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Complex from Homo sap)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Dna (146-mer) (Complex from Synthetic)
PRC2-AEBP2-JARID2 bound to H2AK119ub1 nucleosome
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6wkr
Q-score: 0.362
Kasinath V, Beck C, Sauer P, Poepsel S, Kosmatka J, Faini M, Toso D, Aebersold R, Nogales E
Science (2021) 371 [ Pubmed: 33479123 DOI: doi:10.1126/science.abc3393 ]
- Zinc finger protein aebp2 (33 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- Dna (314-mer) (98 kDa, DNA from Homo sapiens)
- Prc2-aebp2-jarid2 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Histone-lysine n-methyltransferase ezh2 (85 kDa, Protein from Homo sapiens)
- Prc2-aebp2-jarid2 bound to h2ak119ub1 nucleosome (500 kDa, Complex)
- H2ak119ub1 nucleosome (Complex from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Protein jumonji (49 kDa, Protein from Homo sapiens)